Singh K, Ishii T, Parco A, Huang N, Brar D S, Khush G S
International Rice Research Institute, Manila, Philippines.
Proc Natl Acad Sci U S A. 1996 Jun 11;93(12):6163-8. doi: 10.1073/pnas.93.12.6163.
Rice has become a model cereal plant for molecular genetic research. Rice has the most comprehensive molecular linkage maps with more than 2000 DNA markers and shows synteny and colinearity with the maps of other cereal crops. Until now, however, no information was available about the positions of centromeres and arm locations of markers on the molecular linkage map. Secondary and telotrisomics were used to assign restriction fragment length polymorphism markers to specific chromosome arms and thereby to map the positions of centromeres. More than 170 restriction fragment length polymorphism markers were assigned to specific chromosome arms through gene dosage analysis using the secondary and telotrisomics and the centromere positions were mapped on all 12 linkage groups. The orientations of seven linkage groups were reversed to fit the "short arm on top" convention and the corrected map is presented.
水稻已成为分子遗传学研究的模式谷类作物。水稻拥有最全面的分子连锁图谱，包含2000多个DNA标记，并且与其他谷类作物的图谱显示出同线性和共线性。然而，到目前为止，关于分子连锁图谱上着丝粒的位置和标记的臂位置尚无信息。利用次生三体和端体三体将限制性片段长度多态性标记定位到特定染色体臂上，从而绘制着丝粒的位置。通过使用次生三体和端体三体进行基因剂量分析，将170多个限制性片段长度多态性标记定位到特定染色体臂上，并在所有12个连锁群上绘制了着丝粒位置。七个连锁群的方向被反转以符合“短臂在上”的惯例，并给出了校正后的图谱。
