利用单个F2群体构建的具有2275个标记的高密度水稻遗传连锁图谱。
A high-density rice genetic linkage map with 2275 markers using a single F2 population.
作者信息
Harushima Y, Yano M, Shomura A, Sato M, Shimano T, Kuboki Y, Yamamoto T, Lin S Y, Antonio B A, Parco A, Kajiya H, Huang N, Yamamoto K, Nagamura Y, Kurata N, Khush G S, Sasaki T
机构信息
Rice Genome Research Program, National Institute of Agrobiological Resources/Institute of Society for Techno-Innovation of Agriculture, Forestry, and Fisheries, Tsukuba, Ibaraki, Japan.
出版信息
Genetics. 1998 Jan;148(1):479-94. doi: 10.1093/genetics/148.1.479.
A 2275-marker genetic map of rice (Oryza sativa L.) covering 1521.6 cM in the Kosambi function has been constructed using 186 F2 plants from a single cross between the japonica variety Nipponbare and the indica variety Kasalath. The map provides the most detailed and informative genetic map of any plant. Centromere locations on 12 linkage groups were determined by dosage analysis of secondary and telotrisomics using > 130 DNA markers located on respective chromosome arms. A limited influence on meiotic recombination inhibition by the centromere in the genetic map was discussed. The main sources of the markers in this map were expressed sequence tag (EST) clones from Nipponbare callus, root, and shoot libraries. We mapped 1455 loci using ESTs; 615 of these loci showed significant similarities to known genes, including single-copy genes, family genes, and isozyme genes. The high-resolution genetic map permitted us to characterize meiotic recombinations in the whole genome. Positive interference of meiotic recombination was detected both by the distribution of recombination number per each chromosome and by the distribution of double crossover interval lengths.
利用粳稻品种日本晴和籼稻品种卡萨拉斯之间单交产生的186个F2植株，构建了一个覆盖1521.6厘摩（Kosambi函数）的包含2275个标记的水稻（Oryza sativa L.）遗传图谱。该图谱是所有植物中最详细、信息最丰富的遗传图谱。通过使用位于各染色体臂上的130多个DNA标记对次生三体和端三体进行剂量分析，确定了12个连锁群上的着丝粒位置。讨论了遗传图谱中着丝粒对减数分裂重组抑制的有限影响。该图谱中标记的主要来源是来自日本晴愈伤组织、根和茎文库的表达序列标签（EST）克隆。我们利用EST定位了1455个位点；其中615个位点与已知基因有显著相似性，包括单拷贝基因、家族基因和同工酶基因。高分辨率遗传图谱使我们能够对全基因组中的减数分裂重组进行表征。通过每条染色体上重组数的分布以及双交换区间长度的分布，检测到了减数分裂重组的正干涉。
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