Congy N, Mihaesco C
Immunology. 1978 Aug;35(2):307-15.
Goats immunized over 2 months with low doses of 1 mg/kg of dinitrophenylated Salmonella typhimurium responded with low levels of anti-DNP antibodies restricted to the IgM class. The purified antibodies show low association constants (Ka between 10(4) of 10(5) l/M), a high degree of homogeneity (heterogeneity indices alpha between 0.7 and 0.9) and ten combining sites when tested against dinitrophenyl-lysine as ligand by equilibrium dialysis. These binding properties remained unchanged during the whole immune response. When after 9 months the animals received the same immunogen and DNP-BGG, the anti-DNP antibody response included antibodies in the IgG class.
用低剂量（1毫克/千克）二硝基苯基化鼠伤寒沙门氏菌免疫山羊2个多月后，产生的抗二硝基苯酚（anti-DNP）抗体水平较低，且仅限于IgM类。纯化后的抗体显示出较低的结合常数（Ka在10⁴至10⁵升/摩尔之间）、高度的均一性（不均一性指数α在0.7至0.9之间），并且通过平衡透析以二硝基苯基赖氨酸作为配体进行测试时具有十个结合位点。在整个免疫反应过程中，这些结合特性保持不变。9个月后，当动物接受相同的免疫原和二硝基苯酚-牛γ球蛋白（DNP-BGG）时，抗DNP抗体反应包括IgG类抗体。
