[心肌缺血再灌注猪模型中的尿酸生成]

[Urate production in a porcine model of myocardial ischemia-reperfusion].

作者信息

Martín-Jadraque R, Montero C, Mostaza J M, López-Sendón J L, Argomaniz L, Llorente P, Martín-Jadraque L

机构信息

Instituto de Investigaciones Biomédicas del CSIC, Universidad Autónoma de Madrid.

出版信息

Rev Esp Cardiol. 1996 Apr;49(4):281-7.

PMID:8650405

Abstract

OBJECTIVE

This study was designed to investigate urate production by swine hearts using an in vivo regionally ischemic-reperfused model.

ANIMALS AND METHODS

Ten female pigs underwent 60 minutes of myocardial ischemia by clamping of the left anterior descending artery and afterwards 120 minutes of reperfusion. Epicardial biopsies and blood samples from coronary sinus were taken before ligation, at the end of ischemic period and 5, 30, 60 and 120 minutes upon reperfusion.

RESULTS

During ischemia, tissue levels of ATP and ADP greatly declined with a subsequent increase in the concentration of AMP, inosine and hypoxanthine (33 +/- 12 vs 93 +/- 17, 26 +/- 8 vs 768 +/- 86 and 32 +/- 10 vs 219 +/- 26 nmol/g dry weight, p < 0.01 for each). Despite the great increase in the hypoxanthine levels, uric acid concentration remained constant (69 +/- 9 vs 32 +/- 12 nmol/g dry weight, NS). Hypoxanthine, xanthine and uric acid concentrations increased in blood samples obtained from the coronary sinus at the end of ischemic period (17.99 vs 31.03 nmol/ml, p < 0.01, 0.29 vs 1.45 nmol/ml, p < 0.05 and 1.20 vs 2.31 nmol/ml, p < 0.01 respectively) and were enhanced upon reperfusion (35.8 and 3.89 nmol/ml for hypoxanthine and uric acid respectively, p < 0.05) without any significant modifications in their concentrations at the arterial level.

CONCLUSION

These results demonstrate that the ischemic-reperfused swine heart produces urate probably outside the myocardium.

摘要

目的

本研究旨在利用体内局部缺血再灌注模型研究猪心脏尿酸生成情况。

动物与方法

10只雌性猪通过结扎左前降支动脉进行60分钟心肌缺血，随后再灌注120分钟。在结扎前、缺血期末以及再灌注后5、30、60和120分钟采集心外膜活检组织和冠状窦血样。

结果

缺血期间，ATP和ADP的组织水平大幅下降，随后AMP、肌苷和次黄嘌呤浓度升高（分别为33±12对93±17、26±8对768±86、32±10对219±26 nmol/g干重，每组p<0.01）。尽管次黄嘌呤水平大幅升高，但尿酸浓度保持恒定（69±9对32±12 nmol/g干重，无显著性差异）。缺血期末从冠状窦采集的血样中次黄嘌呤、黄嘌呤和尿酸浓度升高（分别为17.99对31.03 nmol/ml，p<0.01；0.29对1.45 nmol/ml，p<0.05；1.20对2.31 nmol/ml，p<0.01），再灌注时进一步升高（次黄嘌呤和尿酸分别为35.8和3.89 nmol/ml，p<0.05），而动脉水平其浓度无显著变化。