Sewell H F, Gell P G, Basu M K
Int Arch Allergy Appl Immunol. 1979;58(4):414-25. doi: 10.1159/000232221.
The effects on the immune response of daily feeding of 30 mg of human serum albumin to rats have been studied. Feeding for periods of 17-20 days consistently resulted in a specific systemic hyporesponsiveness evident on subsequent parenteral immunogen challenge. Local secretory sites such as the major salivary glands were not made hyporesponsive as evidenced by the salivary antibody titres and the enumeration of glandular plaque-forming cells. The levels and classes of antibodies present in the secretions and sera were identified by passive haemagglutination, and a sensitive red-cell-linked antigen-antiglobulin reaction. By the use of radioimmunoassay and haemagglutination inhibition assays it was possible to quantitate the amount of antigen appearing in the circulation at varying times after feeding. It was shown in the use of oesophagectomised rats that absorption of small amounts of intact protein occurs from undefined sites in the oral cavity. Attempts were made to transfer the specific systemic hyporesponsiveness to syngeneic animals using spleen cell and serum transfers.
研究了每天给大鼠喂食30毫克人血清白蛋白对其免疫反应的影响。连续17 - 20天喂食白蛋白，随后进行非肠道免疫原激发时，始终会导致特异性全身性低反应性。主要唾液腺等局部分泌部位并未出现低反应性，这可通过唾液抗体滴度和腺泡斑块形成细胞计数得以证明。通过被动血凝反应以及一种灵敏的红细胞连接抗原 - 抗球蛋白反应，确定了分泌物和血清中存在的抗体水平和类别。利用放射免疫测定法和血凝抑制测定法，能够对喂食后不同时间出现在循环系统中的抗原量进行定量。利用食管切除大鼠的实验表明，口腔中未明确的部位会吸收少量完整蛋白质。尝试通过脾细胞和血清转移，将特异性全身性低反应性转移至同基因动物身上。
