Rupani S P, Gu M B, Konstantinov K B, Dhurjati P S, Van Dyk T K, LaRossa R A
Department of Chemical Engineering, University of Delaware, Newark 19716, USA.
Biotechnol Prog. 1996 May-Jun;12(3):387-92. doi: 10.1021/bp960015u.
The effects of temperature, growth stage, and inducer (ethanol) concentration on the kinetics and magnitude of the stress response were investigated by using an Escherichia coli strain with the grpE heat shock promoter fused to the Vibrio fischeri lux genes. When stressed, the cells responded by changing the level of specific light emission, which was measured both on- and off-line. These measurements were used to characterize and optimize the sensitivity of the construct by determining the conditions at which the culture exhibited maximum specific bioluminescence and minimum response time to ethanol induction in batch cultivation. The results of the batch study were then applied to continuous cultivation, and the effect of dilution rate was determined. These results are of considerable interest in the development of an on-line biological sensor system for the detection and toxicity assessment of chemical pollutants.
通过使用一种将grpE热休克启动子与费氏弧菌lux基因融合的大肠杆菌菌株，研究了温度、生长阶段和诱导剂（乙醇）浓度对应激反应动力学和强度的影响。受到应激时，细胞通过改变特定发光水平做出反应，该发光水平通过在线和离线方式进行测量。这些测量用于通过确定培养物在分批培养中表现出最大比生物发光和对乙醇诱导的最短响应时间的条件，来表征和优化构建体的灵敏度。然后将分批研究的结果应用于连续培养，并确定稀释率的影响。这些结果对于开发用于检测和评估化学污染物毒性的在线生物传感器系统具有重要意义。
