一种使用重组生物发光大肠杆菌细胞检测毒性的微型生物反应器。

A miniature bioreactor for sensing toxicity using recombinant bioluminescent Escherichia coli cells.

作者信息

Gu M B, Dhurjati P S, Van Dyk T K, LaRossa R A

机构信息

Department of Chemical Engineering, University of Delaware, Newark 19716, USA.

出版信息

Biotechnol Prog. 1996 May-Jun;12(3):393-7. doi: 10.1021/bp9600142.

DOI:10.1021/bp9600142

PMID:8652123

Abstract

A miniature bioreactor was fabricated as a contactor between biosensing cells and toxic materials. This miniature bioreactor (58 mL working volume) showed performance similar to that of a conventional bioreactor, as well as the advantages of easy installation, facile operation, and small medium requirements during long-term continuous operation. A performance evaluation measured the response to ethanol in continuous operation by using a recombinant bioluminescent Escherichia coli strain. Continuous cultures were repeatedly induced by the ethanol challenge. Steady-state cell concentrations (OD) were found to be decreased, the induced specific bioluminescence (SBL) peak value was found to be increased, and the peak response time, which is the time constant of this continuous monitoring system, was found to be decreased with increasing dilution rate. Finally on- and off-line bioluminescence monitoring was shown to be reliable, suggesting that this system is suitable for applications such as monitoring the influent and effluent streams of waste water biotreatment plants.

摘要

一种微型生物反应器被制作为生物传感细胞与有毒物质之间的接触装置。这种微型生物反应器（工作体积为58毫升）表现出与传统生物反应器相似的性能，以及易于安装、操作简便和长期连续运行期间培养基需求量小的优点。性能评估通过使用重组生物发光大肠杆菌菌株来测量连续运行中对乙醇的响应。通过乙醇刺激反复诱导连续培养。发现稳态细胞浓度（OD）降低，诱导的比生物发光（SBL）峰值增加，并且作为该连续监测系统时间常数的峰值响应时间随着稀释率的增加而减少。最后，在线和离线生物发光监测被证明是可靠的，这表明该系统适用于监测废水生物处理厂的进水和出水等应用。