Mandel M A, Feldmann K A, Herrera-Estrella L, Rocha-Sosa M, León P
Departamento de Ingeniería Genética de Plantas, CINVESTAV, México.
Plant J. 1996 May;9(5):649-58. doi: 10.1046/j.1365-313x.1996.9050649.x.
An albino mutant designated cla1-1 (for "cloroplastos alterados', or "altered chloroplasts') has been isolated from a T-DNA-generated library of Arabidopsis thaliana. In cla1-1 plants, chloroplast development is arrested at an early stage. cla1-1 plants behave like wild-type in their capacity to etiolate and produce anthocyanins indicating that the light signal transduction pathway seems to be unaffected. Genetic and molecular analyses show that the disruption of a single gene, CLA1, by the T-DNA insertion is responsible for the mutant phenotype. RNA expression patterns indicate that CLA1 is positively regulated by light and that it has different effects on the steady-state RNA levels of some nuclear- and chloroplast-encoded photosynthetic genes. Although the specific function of the CLA1 gene is still unknown, it encodes a novel protein conserved in evolution between photosynthetic bacteria and plants which is essential for chloroplast development in Arabidopsis.
从拟南芥的一个由T-DNA构建的文库中分离出一个白化突变体，命名为cla1-1（“cloroplastos alterados”的缩写，意为“叶绿体改变”）。在cla1-1植株中，叶绿体发育在早期阶段就停滞了。cla1-1植株在黄化和产生花青素的能力方面表现得与野生型相似，这表明光信号转导途径似乎未受影响。遗传和分子分析表明，T-DNA插入导致单个基因CLA1的破坏是造成突变体表型的原因。RNA表达模式表明CLA1受光正向调控，并且它对一些核编码和叶绿体编码的光合基因的稳态RNA水平有不同影响。尽管CLA1基因的具体功能仍然未知，但它编码一种在光合细菌和植物之间进化上保守的新型蛋白质，这对拟南芥中的叶绿体发育至关重要。
