Tsujita T, Morimoto C, Okuda H
Second Department of Medical Biochemistry, School of Medicine, Ehirne University, Japan.
Obes Res. 1995 Dec;3 Suppl 5:633S-636S. doi: 10.1002/j.1550-8528.1995.tb00478.x.
Sonication of rat fat cells caused an increase in lipolysis in the absence of lipolytic hormones (basal lipolysis) and loss of epinephrine responsiveness. Sonication of endogenous lipid droplets from fat cells also induced an increase in lipolysis in the presence of HSL. Addition of phosphatidylcholine to the sonicated lipid droplets reduced the hydrolysis of triglyceride by HSL. These results suggest that the active HSL is already present in the fat cell even in the absence of lipolytic hormone, and phosphatidylcholine on the surface of endogenous lipid droplets causes inhibition toward lipolytic action of HSL. The decrease in the surface phosphatidylcholine concentration in endogenous lipid droplets was proved to cause the increase in basal lipolysis. It is demonstrated that basal lipolysis was elevated in the enlarged fat cells of obese rats by reduction of surface phosphatidylcholine concentration of the endogenous lipid droplets.
在没有脂解激素（基础脂解）的情况下，对大鼠脂肪细胞进行超声处理会导致脂解增加以及肾上腺素反应性丧失。对脂肪细胞内源性脂滴进行超声处理，在存在激素敏感脂肪酶（HSL）的情况下也会诱导脂解增加。向超声处理的脂滴中添加磷脂酰胆碱可减少HSL对甘油三酯的水解。这些结果表明，即使在没有脂解激素的情况下，活性HSL也已存在于脂肪细胞中，内源性脂滴表面的磷脂酰胆碱会抑制HSL的脂解作用。内源性脂滴表面磷脂酰胆碱浓度的降低被证明会导致基础脂解增加。结果表明，肥胖大鼠增大的脂肪细胞中，内源性脂滴表面磷脂酰胆碱浓度降低，基础脂解升高。
