Yun C H, Estrada A, Gajadhar A A, Redmond M J, Laarveld B
Department of Animal and Poultry Science, University of Saskatchewan, Saskatoon, Canada.
Comp Immunol Microbiol Infect Dis. 1996 Jan;19(1):39-46. doi: 10.1016/0147-9571(95)00019-4.
The effect of in vivo immunoneutralization of somatostatin (SRIF) on Eimeria vermiformis intestinal infection was studied in resistant (BALB/c), and susceptible (C57BL/6) mouse strains. An anti-SRIF monoclonal antibody (MAb-SRIF) was used to passively immunize the mice by intraperitoneal injection. The animals were subsequently orally infected with oocysts of E. vermiformis. Individual fecal samples were collected daily for 21 days to monitor the kinetics of oocyst shedding. The fecal oocyst shedding was significantly higher in the C57BL/6 strain than in the BALB/c strain (P < 0.01). Passive immunization with MAb-SRIF in the C57BL/6 mice significantly reduced the number of oocysts in feces (P < 0.05), when compared to the infected non-immunized mice of the same strain. Infected BALB/c mice showed no difference in oocyst shedding in response to the passive immunoneutralization with MAb-SRIF. In conclusion, passive immunization with MAb-SRIF increased resistance to E. vermiformis-infection in the susceptible C57BL/6 mice, but not in the resistant BALB/c mice. This suggests that SRIF modulates gut immune function in parasitic infection.
在抗性（BALB/c）和易感（C57BL/6）小鼠品系中研究了体内免疫中和生长抑素（SRIF）对蠕形艾美耳球虫肠道感染的影响。使用抗SRIF单克隆抗体（MAb-SRIF）通过腹腔注射对小鼠进行被动免疫。随后给动物口服感染蠕形艾美耳球虫的卵囊。连续21天每天收集个体粪便样本，以监测卵囊排出的动力学。C57BL/6品系的粪便卵囊排出量显著高于BALB/c品系（P < 0.01）。与相同品系的未免疫感染小鼠相比，用MAb-SRIF对C57BL/6小鼠进行被动免疫显著减少了粪便中的卵囊数量（P < 0.05）。感染的BALB/c小鼠在接受MAb-SRIF被动免疫中和后，卵囊排出没有差异。总之，用MAb-SRIF进行被动免疫可增加易感C57BL/6小鼠对蠕形艾美耳球虫感染的抵抗力，但对抗性BALB/c小鼠无效。这表明SRIF在寄生虫感染中调节肠道免疫功能。
