Wallach M, Pillemer G, Yarus S, Halabi A, Pugatsch T, Mencher D
Kuvin Center for the Study of Infectious and Tropical Diseases, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Infect Immun. 1990 Feb;58(2):557-62. doi: 10.1128/iai.58.2.557-562.1990.
Eimeria maxima gametocytes contain two major antigens with molecular masses of 56 and 82 kilodaltons (kDa) which are recognized by convalescent sera from immune chickens. Preparations enriched in these two antigens were used to immunize mice, and several monoclonal antibodies which specifically reacted with the 56-kDa antigen were produced. One of these monoclonal antibodies of the immunoglobulin M subclass, along with immune chicken sera raised against affinity-purified 56- and 82-kDa antigens, was used to passively immunize chicks. On the basis of the parameter of total oocyst output, it was found that these antibodies provided partial protection (40 to 50% inhibition) against E. maxima challenge infections.
巨型艾美耳球虫配子体含有两种主要抗原，分子量分别为56和82千道尔顿（kDa），可被免疫鸡的恢复期血清识别。富含这两种抗原的制剂用于免疫小鼠，并产生了几种与56-kDa抗原特异性反应的单克隆抗体。其中一种免疫球蛋白M亚类的单克隆抗体，与针对亲和纯化的56-kDa和82-kDa抗原产生的免疫鸡血清一起，用于被动免疫雏鸡。根据总卵囊产量参数，发现这些抗体对巨型艾美耳球虫攻击感染提供了部分保护（抑制率为40%至50%）。
