Platt A, Langdon T, Arst H N, Kirk D, Tollervey D, Sanchez J M, Caddick M X
Department of Genetics and Microbiology, Donnan Laboratories, University of Liverpool, Liverpool, UK.
EMBO J. 1996 Jun 3;15(11):2791-801.
AREA is a GATA transcription factor which mediates nitrogen metabolite repression in Aspergillus nidulans in response to intracellular glutamine levels. We have identified and localized three elements important to modulation of AREA function: a region of 13 residues within the DNA-binding GATA domain which forms a putative extended loop structure, the 12 C-terminal residues, and sequences within a 218 nucleotide region of the 3' UTR. The 12 C-terminal residues are also required for transcriptional activation at a subset of loci under areA control. Specific deletions within the 3' UTR and the C-terminus cause similar levels of derepression and the mutations are additive, implicating two principal signal transduction pathways. The contribution of the 3' UTR to AREA modulation is effected at the level of transcript stability such that the areA mRNA is at least five times more stable under nitrogen-derepressing conditions than it is under repressing growth conditions.
AREA是一种GATA转录因子，它在构巢曲霉中响应细胞内谷氨酰胺水平介导氮代谢物阻遏。我们已经鉴定并定位了对AREA功能调节重要的三个元件：DNA结合GATA结构域内13个残基的区域，该区域形成一个假定的延伸环结构；12个C末端残基；以及3' UTR的218个核苷酸区域内的序列。在areA控制下的一部分基因座处进行转录激活也需要这12个C末端残基。3' UTR和C末端内的特定缺失导致相似程度的去阻遏，并且这些突变具有累加性，这暗示了两条主要的信号转导途径。3' UTR对AREA调节的贡献是在转录本稳定性水平上实现的，使得areA mRNA在氮去阻遏条件下的稳定性至少是在阻遏生长条件下的五倍。
