Robinson J P, Derreberry S, Liddle R A, Ascoli M, Puett D
Mol Cell Biochem. 1977 Mar 21;15(1):63-6. doi: 10.1007/BF01731289.
Nephrectomy of mature rats was found to result in a significant increase in the circulatory half-life of tritiated ovine lutropin. The interaction of the glycoprotein hormone with the kidneys was studied in a more direct fashion using electron microscopic autoradiography. Evidence is presented showing the transfer of the hormone from microvilli into tubular epithelia (probably via vesicular transport), where radioactivity then becomes associated with lysosomes. This provides direct support for related results based on subcellular fractionation in which renal lysosomal catabolism was suggested as being important in the degradation of tritiated lutropin (M. Ascoli, R. A. Liddle, and D. Puett, Molecular and Cellular Endocrinology 4, 297, 1976). These results add substantial weight to the growing evidence that the kidneys assume a major role in controlling the concentration of circulating macromolecules.
已发现切除成年大鼠的肾脏会导致氚标记的绵羊促黄体激素的循环半衰期显著增加。使用电子显微镜放射自显影术以更直接的方式研究了糖蛋白激素与肾脏的相互作用。有证据表明，激素从微绒毛转移到肾小管上皮细胞（可能通过囊泡运输），然后放射性与溶酶体相关联。这为基于亚细胞分级分离的相关结果提供了直接支持，在该分级分离中，肾溶酶体分解代谢被认为在氚标记的促黄体激素的降解中起重要作用（M. 阿斯克利、R. A. 利德尔和D. 普埃特，《分子与细胞内分泌学》4，297，1976）。这些结果进一步有力地证明了越来越多的证据，即肾脏在控制循环大分子浓度方面发挥着主要作用。
