Walker J A, Harmon D L
Department of Animal Sciences, University of Kentucky, Lexington 40546-0215, USA.
J Anim Sci. 1995 Dec;73(12):3766-74. doi: 10.2527/1995.73123766x.
Seven Holstein steers (234 +/- 6.4 kg) surgically fitted with pancreatic cannulas, ruminal and abomasal infusion cannulas, and hepatic-portal vein catheters were used in a 4 x 7 incomplete Latin square design experiment to examine the influence of ruminal and abomasal carbohydrate infusion on enzyme secretion and composition of pancreatic juice. Four treatments were arranged in a 2 x 2 factorial: 1) ruminal starch hydrolysate (SH; 34.2 g/[h.site]) or abomasal water and 2) abomasal SH or ruminal water. Starch hydrolysate is raw cornstarch that has been partially digested by a heat-stable alpha-amylase. Experimental periods were 14 d with 9 to 10 d of adaptation, 4 d of pancreatic collection, and blood collection on d 14. Abomasal SH infusion tended (P < .10) to increase pancreatic fluid secretion. The pH of pancreatic juice was higher (P < .01) for ruminal SH infusion, and abomasal SH infusion tended (P < .10) to result in lower pH of pancreatic juice. alpha-Amylase concentrations (units/milliliter and units/milligram of protein) and secretion (units/hour) were less (P < .002) for abomasal SH infusion. Chymotrypsin concentration (units/liter; P < .01) and secretion (units/hour; P < .10) were less for abomasal SH infusion; however, a rumen x abomasal interaction was found (P < .05). Chloride concentration (milligrams/deciliter) and secretion (milligrams/hour) were increased (P < .01) for abomasal SH infusion. Abomasal SH infusion resulted in increased (P < .01) portal blood glucose concentrations; however, portal plasma insulin concentration was not affected (P > .10). Abomasal SH infusion altered alpha-amylase secretion in steers, but ruminal SH infusion had minimal effect on alpha-amylase secretion. These changes suggest abosamal infusion of SH may negatively impact secretion of pancreatic alpha-amylase.
选用7头荷斯坦公牛（体重234±6.4千克），通过手术安装胰管插管、瘤胃和真胃灌注插管以及肝门静脉导管，采用4×7不完全拉丁方设计试验，研究瘤胃和真胃碳水化合物灌注对胰液酶分泌及成分的影响。试验采用2×2析因设计安排4种处理：1）瘤胃淀粉水解产物（SH；34.2克/[小时·位点]）或真胃灌注水，2）真胃灌注SH或瘤胃灌注水。淀粉水解产物是由热稳定α-淀粉酶部分消化的生玉米淀粉。试验期为14天，其中9至10天用于适应，4天用于收集胰液，并在第14天采集血液。真胃灌注SH有增加胰液分泌的趋势（P<0.10）。瘤胃灌注SH时胰液的pH值较高（P<0.01），真胃灌注SH有使胰液pH值降低的趋势（P<0.10）。真胃灌注SH时，α-淀粉酶浓度（单位/毫升和单位/毫克蛋白质）和分泌量（单位/小时）较低（P<0.002）。真胃灌注SH时，胰凝乳蛋白酶浓度（单位/升；P<0.01）和分泌量（单位/小时；P<0.10）较低；然而，发现瘤胃和真胃存在交互作用（P<0.05）。真胃灌注SH时，氯离子浓度（毫克/分升）和分泌量（毫克/小时）增加（P<0.01）。真胃灌注SH导致门静脉血糖浓度升高（P<0.01）；然而，门静脉血浆胰岛素浓度未受影响（P>0.10）。真胃灌注SH改变了公牛的α-淀粉酶分泌，但瘤胃灌注SH对α-淀粉酶分泌影响极小。这些变化表明，真胃灌注SH可能对胰腺α-淀粉酶的分泌产生负面影响。
