Wild J, Rossmeissl P, Walter W A, Gross C A
Department of Bacteriology, University of Wisconsin-Madison, 53706, USA.
J Bacteriol. 1996 Jun;178(12):3608-13. doi: 10.1128/jb.178.12.3608-3613.1996.
We used depletion studies designed to further investigate the role of the DnaK, DnaJ, and GrpE heat shock proteins in the SecB-dependent and SecB-independent secretion pathways. Our previous finding that SecB-deficient strains containing the grpE280 mutation were still secretion proficient raised the possibility that GrpE was not involved in this secretory pathway. Using depletion studies, we now demonstrate a requirement for GrpE in this pathway. In addition, depletion studies demonstrate that while DnaK, DnaJ, and GrpE are involved in the secretion of the SecB-independent proteins (alkaline phosphatase, ribose-binding protein, and beta-lactamase), they are not the primary chaperones in this process.
我们采用了消耗性研究，旨在进一步探究DnaK、DnaJ和GrpE热休克蛋白在SecB依赖性和SecB非依赖性分泌途径中的作用。我们之前发现，含有grpE280突变的SecB缺陷菌株仍具有分泌能力，这增加了GrpE不参与该分泌途径的可能性。通过消耗性研究，我们现在证明了该途径中对GrpE的需求。此外，消耗性研究表明，虽然DnaK、DnaJ和GrpE参与了SecB非依赖性蛋白（碱性磷酸酶、核糖结合蛋白和β-内酰胺酶）的分泌，但它们并非此过程中的主要伴侣蛋白。
