突变型DnaK伴侣蛋白导致大肠杆菌核糖体组装缺陷。
Mutant DnaK chaperones cause ribosome assembly defects in Escherichia coli.
作者信息
Alix J H, Guérin M F
机构信息
Institut de Biologie Physico-Chimique, Paris, France.
出版信息
Proc Natl Acad Sci U S A. 1993 Oct 15;90(20):9725-9. doi: 10.1073/pnas.90.20.9725.
Abstract
To determine whether the biogenesis of ribosomes in Escherichia coli is the result of the self-assembly of their different constituents or involves the participation of additional factors, we have studied the influence of a chaperone, the product of the gene dnaK, on ribosome assembly in vivo. Using three thermosensitive (ts) mutants carrying the mutations dnaK756-ts, dnaK25-ts, and dnaK103-ts, we have observed the accumulation at nonpermissive temperature (45 degrees C) of ribosomal particles with different sedimentation constants--namely, 45S, 35S, and 25S along with the normal 30S and 50S ribosomal subunits. This is the result of a defect not in thermostability but in ribosome assembly at the nonpermissive temperature. These abnormal ribosomal particles are rescued if the mutant cells are returned to 30 degrees C. Thus, the product of the dnaK gene is implicated in ribosome biogenesis at high temperature.
摘要
为了确定大肠杆菌中核糖体的生物合成是其不同组分自我组装的结果，还是涉及其他因素的参与，我们研究了一种伴侣蛋白（基因dnaK的产物）对体内核糖体组装的影响。利用携带dnaK756-ts、dnaK25-ts和dnaK103-ts突变的三个温度敏感（ts）突变体，我们观察到在非允许温度（45℃）下，具有不同沉降常数的核糖体颗粒的积累——即45S、35S和25S，以及正常的30S和50S核糖体亚基。这是由于在非允许温度下核糖体组装存在缺陷，而非热稳定性缺陷所致。如果将突变细胞恢复到30℃，这些异常的核糖体颗粒就能得到挽救。因此，dnaK基因的产物与高温下的核糖体生物合成有关。
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