在大肠杆菌中，H-NS通过micF反义RNA调控OmpF的表达。
H-NS regulates OmpF expression through micF antisense RNA in Escherichia coli.
作者信息
Suzuki T, Ueguchi C, Mizuno T
机构信息
Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University, Japan.
出版信息
J Bacteriol. 1996 Jun;178(12):3650-3. doi: 10.1128/jb.178.12.3650-3653.1996.
Abstract
H-NS is a major constituent of the Escherichia coli nucleoid. Expression of the major outer membrane proteins, OmpC and OmpF, is influenced by hns mutations such that OmpC expression increases whereas OmpF expression decreases irrespective of the osmolarity of the medium (K. A. Graeme-Cook, G. May, E. Bremer, and C. F. Higgins, Mol. Microbiol. 3:1287-1294, 1989). In this study we show that the effect of an hns::neo mutation on OmpF expression is largely diminished in a deletion mutant carrying the micF gene that encodes the ompF mRNA-specific antisense RNA. In addition, the micF transcript levels in the hns::neo mutation are high compared with transcript levels in wild-type cells. On the basis of these results, we provide evidence for a link between OmpC/OmpF expression and the regulatory function of H-NS. We suggest that H-NS most likely affects OmpC expression directly at the level of transcription, but OmpF expression is indirectly regulated by micF antisense RNA.
摘要
H-NS是大肠杆菌类核的主要组成部分。主要外膜蛋白OmpC和OmpF的表达受hns突变影响，无论培养基的渗透压如何，OmpC表达增加而OmpF表达减少（K. A. Graeme-Cook、G. May、E. Bremer和C. F. Higgins，《分子微生物学》3:1287 - 1294，1989）。在本研究中我们表明，在携带编码ompF mRNA特异性反义RNA的micF基因的缺失突变体中，hns::neo突变对OmpF表达的影响大大减弱。此外，与野生型细胞中的转录本水平相比，hns::neo突变中的micF转录本水平较高。基于这些结果，我们提供了OmpC/OmpF表达与H-NS调节功能之间存在联系的证据。我们认为H-NS很可能在转录水平直接影响OmpC表达，但OmpF表达是由micF反义RNA间接调节的。
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