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一种调控基因表达的独特机制：由互补RNA转录本（micRNA）介导的翻译抑制。

A unique mechanism regulating gene expression: translational inhibition by a complementary RNA transcript (micRNA).

作者信息

Mizuno T, Chou M Y, Inouye M

出版信息

Proc Natl Acad Sci U S A. 1984 Apr;81(7):1966-70. doi: 10.1073/pnas.81.7.1966.

DOI:10.1073/pnas.81.7.1966
PMID:6201848
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC345417/
Abstract

The expression of the genes for the major outer membrane proteins OmpF and OmpC are osmoregulated. The ompC locus was found to be transcribed bidirectionally under conditions of high osmolarity and a 174-base transcript encoded upstream of ompC was found to inhibit the OmpF production and to substantially reduce the amount of the ompF mRNA. This RNA [mRNA-interfering complementary RNA (micRNA)] has a long sequence that is complementary to the 5' end region of the ompF mRNA. We propose that the micRNA inhibits the translation of the ompF mRNA by hybridizing with it. This RNA interaction may cause premature termination of the transcription of the ompF gene or destabilization of the ompF mRNA or both.

摘要

主要外膜蛋白OmpF和OmpC的基因表达受渗透压调节。发现在高渗透压条件下ompC基因座双向转录，并且在ompC上游编码的一个174个碱基的转录物被发现抑制OmpF的产生并大幅减少ompF mRNA的量。这种RNA[mRNA干扰互补RNA（micRNA）]具有与ompF mRNA的5'末端区域互补的长序列。我们提出micRNA通过与ompF mRNA杂交来抑制其翻译。这种RNA相互作用可能导致ompF基因转录的提前终止或ompF mRNA的不稳定或两者皆有。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9fa/345417/cc96abfe37ad/pnas00608-0045-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9fa/345417/df6ef8f1b868/pnas00608-0044-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9fa/345417/9bbe4fc680b2/pnas00608-0045-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9fa/345417/5b2582d49ce2/pnas00608-0045-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9fa/345417/cc96abfe37ad/pnas00608-0045-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9fa/345417/df6ef8f1b868/pnas00608-0044-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9fa/345417/9bbe4fc680b2/pnas00608-0045-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9fa/345417/5b2582d49ce2/pnas00608-0045-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9fa/345417/cc96abfe37ad/pnas00608-0045-c.jpg

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Genetic analysis of the ompB locus in Escherichia coli K-12.大肠杆菌K-12中ompB基因座的遗传分析。
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