Chan E C, Qiu Y S, Siboo R, Noble P
Department of Microbiology and Immunology, Faculty of Medicine, McGill University, Quebec, Canada.
Oral Microbiol Immunol. 1995 Apr;10(2):122-4. doi: 10.1111/j.1399-302x.1995.tb00131.x.
Using a two-layer system, a bottom layer containing treponema cells suspended in NOS (New Oral Spirochete)-Noble agar medium or NOS-Bacto agar medium and overlaid with cell-free NOS-agarose medium resulted in the spirochete cells migrating into the top layer. However, if the positions of the medium layers were reversed with the cells inoculated into the bottom layer containing NOS-agarose, there was no migration into the upper layer. This suggests migration of the spirochetes away from Bacto and Noble agars. Using a 3-layer system in which cells were inoculated into a middle layer consisting of NOS-agarose medium and sandwiched between cell-free NOS-agarose medium layers, cells remained within the middle layer. If the cells were inoculated into a middle layer consisting of NOS-Bacto agar medium while the upper and lower layers remained unchanged, cells migrated into both upper and lower layers. If cells that had migrated into the upper layer were transferred into a middle layer, they virtually all migrated into the upper layer repeatedly. Cells that had migrated into the lower layer and transferred to the middle layer migrated repeatedly into the lower layer. These results suggest the possible existence of two distinct locomotory phenotypes within this strain of treponeme.
使用双层系统时，底层含有悬浮于新口腔螺旋体-诺布尔琼脂培养基或新口腔螺旋体-细菌琼脂培养基中的密螺旋体细胞，上面覆盖无细胞的新口腔螺旋体-琼脂糖培养基，结果螺旋体细胞迁移到了上层。然而，如果培养基层的位置颠倒，将细胞接种到含有新口腔螺旋体-琼脂糖的底层，则不会向上层迁移。这表明螺旋体从细菌琼脂和诺布尔琼脂中迁移出来。使用三层系统，将细胞接种到由新口腔螺旋体-琼脂糖培养基组成的中间层，并夹在无细胞的新口腔螺旋体-琼脂糖培养基层之间，细胞仍留在中间层。如果将细胞接种到由新口腔螺旋体-细菌琼脂培养基组成的中间层，而上下层保持不变，细胞会向上层和下层迁移。如果已经迁移到上层的细胞被转移到中间层，它们几乎都会反复迁移到上层。已经迁移到下层并转移到中间层的细胞会反复迁移到下层。这些结果表明，在这种密螺旋体菌株中可能存在两种不同的运动表型。
