Harris A W, Walker M M, Smolka A, Waller J M, Baron J H, Misiewicz J J
Parkside Helicobacter Study Group, Central Middlesex and St Mary's Hospitals, London.
J Clin Pathol. 1996 Apr;49(4):309-12. doi: 10.1136/jcp.49.4.309.
To investigate the prevalence, and relation to Helicobacter pylori, of parietal cells in the duodenal bulb using a monoclonal antibody directed against H+,K(+)-ATPase (HK12.18).
Twenty six patients with duodenal ulcer disease and 16 healthy controls were studied. H pylori status was determined by gastric histology and culture and by the 13C-urea breath test. Four biopsy specimens were taken from the duodenal bulb and stained with HK12.18. The presence/absence and number of parietal cells in the duodenal bulb were assessed blindly by a histopathologist.
The overall prevalence of parietal cells in the duodenal bulb was 31% (13/42) and was similar in patients with duodenal ulcer and in controls, and in H pylori positive and negative subjects. The median (range) number of parietal cells in the duodenal bulb was 7.5 (4-20) parietal cells/subject, and was similar in all four groups.
The prevalence of parietal cells in the duodenal bulb (31%) is notably higher than previously reported in endoscopic studies, and is in keeping with reports from studies on necropsy/operative specimens. There was no difference in the prevalence or number of parietal cells in the duodenal bulb between patients with duodenal ulcer and controls, regardless of H pylori status. These findings suggest that parietal cells in the duodenal bulb do not contribute to the pathogenesis of duodenal ulcer.
使用针对H⁺，K⁺-ATP酶（HK12.18）的单克隆抗体，研究十二指肠球部壁细胞的患病率及其与幽门螺杆菌的关系。
对26例十二指肠溃疡病患者和16名健康对照者进行研究。通过胃组织学检查、培养以及¹³C-尿素呼气试验确定幽门螺杆菌感染状况。从十二指肠球部取4份活检标本，用HK12.18染色。由一名组织病理学家在不知情的情况下评估十二指肠球部壁细胞的有无及数量。
十二指肠球部壁细胞的总体患病率为31%（13/42），在十二指肠溃疡患者和对照组以及幽门螺杆菌阳性和阴性受试者中相似。十二指肠球部壁细胞的中位数（范围）数量为7.5（4 - 20）个/受试者，在所有四组中相似。
十二指肠球部壁细胞的患病率（31%）明显高于先前内镜研究报告的结果，与尸检/手术标本研究报告一致。无论幽门螺杆菌感染状况如何，十二指肠溃疡患者和对照组十二指肠球部壁细胞的患病率或数量均无差异。这些发现表明十二指肠球部壁细胞与十二指肠溃疡的发病机制无关。
