Kemp E B, Belshe R B, Hoft D F
National Institute of Allergy and Infectious Diseases Vaccine, Saint Louis University Health Sciences Center, Missouri, USA.
J Infect Dis. 1996 Jul;174(1):113-9. doi: 10.1093/infdis/174.1.113.
Healthy volunteers were randomized to receive percutaneous or intradermal bacille Calmette-Guérin (BCG) vaccination. Delayed-type hypersensitivity (DTH) to tuberculin, as well as proliferative and interferon-gamma responses in peripheral blood mononuclear cells stimulated by whole cell lysates and culture filtrates of Mycobacterium tuberculosis and Mycobacterium avium-intracellulare, were compared before and after vaccination. Positive DTH reactions were detected in 83% of intradermal and 40% of percutaneous BCG recipients 3 months after vaccination (P < .004). M. tuberculosis-specific proliferation was increased after intradermal BCG (P < .01) but not after percutaneous BCG compared with prevaccination responses. In addition, M. tuberculosis-specific interferon-gamma production was increased after intradermal BCG compared with both prevaccination responses (P < .04) and those measured after percutaneous BCG (P < .05). Predominant immune responses stimulated by BCG were directed against antigens present in mycobacterial whole cell lysate. These results indicate that the BCG vaccination route can affect both in vivo and in vitro immune responses.
健康志愿者被随机分为接受经皮或皮内卡介苗（BCG）接种两组。比较了接种前后对结核菌素的迟发型超敏反应（DTH），以及由结核分枝杆菌和鸟分枝杆菌 - 胞内分枝杆菌的全细胞裂解物和培养滤液刺激的外周血单核细胞中的增殖反应和干扰素 -γ反应。接种疫苗3个月后，83%的皮内接种BCG者和40%的经皮接种BCG者检测到阳性DTH反应（P < 0.004）。与接种前反应相比，皮内接种BCG后结核分枝杆菌特异性增殖增加（P < 0.01），但经皮接种BCG后未增加。此外，与接种前反应（P < 0.04）和经皮接种BCG后测量的反应（P < 0.05）相比，皮内接种BCG后结核分枝杆菌特异性干扰素 -γ产生增加。卡介苗刺激的主要免疫反应针对分枝杆菌全细胞裂解物中存在的抗原。这些结果表明，卡介苗接种途径可影响体内和体外免疫反应。
