Jensen J, Warner T, Johnson C, Balish E
University of Wisconsin Medical School, Department of Surgery, Madison 53706-1532, USA.
J Infect Dis. 1996 Jul;174(1):133-40. doi: 10.1093/infdis/174.1.133.
Oral immunization with live Candida albicans evoked antibody- and cell-mediated immune responses in gnotobiotic C.B-17 and BALB/c mice. No deaths or systemic candidiasis of endogenous origin were evident in these C. albicans-colonized (pure culture) mice. Histologic examination showed minimal to no infection of the stomach, esophagus, or tongue by C. albicans. Not only were orally immunized mice more resistant to systemic candidiasis (intravenous challenge) than were germfree (nonimmunized) controls, but immunity could be transferred to susceptible mice with immune spleen cells. Oral immunization elicited a Th1-type response in spleen cells and a Th2 response in Peyer's patch lymphocytes. The alimentary tracts of these orally immunized mice remained chronically colonized with C. albicans in spite of the presence of both antibody- and cell-mediated immune responses to C. albicans.
用活的白色念珠菌进行口服免疫可在无菌的C.B-17和BALB/c小鼠中引发抗体介导和细胞介导的免疫反应。在这些定殖有白色念珠菌（纯培养物）的小鼠中，未出现内源性起源的死亡或系统性念珠菌病。组织学检查显示，白色念珠菌对胃、食管或舌头的感染轻微或未感染。口服免疫的小鼠不仅比无菌（未免疫）对照更能抵抗系统性念珠菌病（静脉内攻击），而且免疫可通过免疫脾细胞转移至易感小鼠。口服免疫在脾细胞中引发Th1型反应，在派尔集合淋巴结淋巴细胞中引发Th2反应。尽管存在针对白色念珠菌的抗体介导和细胞介导的免疫反应，但这些口服免疫小鼠的消化道仍长期定殖有白色念珠菌。
