Riepenhoff-Talty M, Gouvea V, Evans M J, Svensson L, Hoffenberg E, Sokol R J, Uhnoo I, Greenberg S J, Schäkel K, Zhaori G, Fitzgerald J, Chong S, el-Yousef M, Nemeth A, Brown M, Piccoli D, Hyams J, Ruffin D, Rossi T
Departments of Pediatrics, State University of New York at Buffalo, USA.
J Infect Dis. 1996 Jul;174(1):8-15. doi: 10.1093/infdis/174.1.8.
The purpose of this retrospective study was to examine liver tissue from patients with cholestatic disease for the presence of group C rotavirus RNA. The reverse transcriptase-polymerase chain reaction (PCR) for genes 5 and 6 was used, and the PCR products were subjected to liquid hybridization with a 32P-labeled probe. A second amplification with nested primers was also used. Samples from 32 subjects (20 with biliary atresia or choledochal cyst and 12 controls) were tested. Ten of 20 biliary atresia patients were positive for group C rotavirus RNA; no controls were positive (P < .003). Three of the positive patients were positive for both genes 5 and 6. Six of the 10 had > 1 sample that was positive. These data suggest a possible relationship between group C rotavirus and extrahepatic biliary atresia in the 10 patients in whom virus RNA was detected.
这项回顾性研究的目的是检测胆汁淤积性疾病患者肝脏组织中是否存在C组轮状病毒RNA。采用针对基因5和基因6的逆转录聚合酶链反应（PCR），PCR产物与32P标记的探针进行液相杂交。还使用嵌套引物进行了第二次扩增。检测了32名受试者的样本（20例患有胆道闭锁或胆总管囊肿，12例为对照）。20例胆道闭锁患者中有10例C组轮状病毒RNA呈阳性；对照均为阴性（P <.003）。3例阳性患者的基因5和基因6均呈阳性。10例中有6例不止1份样本呈阳性。这些数据表明，在检测到病毒RNA的10例患者中，C组轮状病毒与肝外胆道闭锁之间可能存在关联。
