Saruta K, Hoshina S, Machida K
Department of Laboratory Medicine, Jikei University School of Medicine, Tokyo, Japan.
Microbiol Immunol. 1995;39(11):839-44. doi: 10.1111/j.1348-0421.1995.tb03280.x.
Staphylococcus aureus is the most predominant and important pathogen in clinical microbiology. A DNA amplification assay using the polymerase chain reaction (PCR) was designed to identify S. aureus through a single-base-pair mismatch in the sequences of staphylococcal 16S ribosomal RNA (16S rRNA) genes. It was able to detect and identify S. aureus without requiring additional analytical techniques. Twenty-eight staphylococcal and non-staphylococcal strains were tested to verify the specificity of the assay, and only S. aureus strains gave a positive reaction. It may be possible to provide immediate and exact information for the identification of S. aureus.
金黄色葡萄球菌是临床微生物学中最主要且最重要的病原体。设计了一种使用聚合酶链反应（PCR）的DNA扩增检测方法，通过葡萄球菌16S核糖体RNA（16S rRNA）基因序列中的单碱基对错配来鉴定金黄色葡萄球菌。该方法无需额外的分析技术就能检测和鉴定金黄色葡萄球菌。对28株葡萄球菌和非葡萄球菌菌株进行了测试以验证该检测方法的特异性，只有金黄色葡萄球菌菌株呈现阳性反应。它可能为金黄色葡萄球菌的鉴定提供即时且准确的信息。
