Ochudło S, Wajgt A
Katedry i Kliniki Neurologii Slaskiej Akademii Medycznej w Katowicach.
Neurol Neurochir Pol. 1996 Jan-Feb;30(1):7-16.
The effects of treatment with cyclophosphamide (CY) and prednisone on the spectrum of oligoclonal IgG (olgG) of the cerebrospinal fluid were studied in 33 cases of slowly progressing multiple sclerosis. CY was administered intravenously in a total dose of 4 g during 10 days. This treatment was supplemented with prednisone 775 mg p.o. given during the following 25 days. The electrophoretic separation of proteins in non-concentrated CSF and in serum before and immediately after the treatment was done by Laemili's method with associated impregnation with silver salts. The analysis of these electrophorograms showed the presence of qualitative and quantitative changes of olgG spectrum. Quantitative changes found in 59% of the cases included more pronounced (8% of cases) appearance or attenuation (51% of cases) of individual bands. Qualitative changes in form of elimination of bands were noted in 29% of cases, and in 12% of cases new bands appeared. The authors conclude that, the results confirm a strong immunosupressive effect of CY on olgG in slowly progressing m.s.
在33例缓慢进展型多发性硬化症患者中，研究了环磷酰胺（CY）和泼尼松治疗对脑脊液寡克隆IgG（olgG）谱的影响。CY静脉给药，10天内总剂量为4g。在接下来的25天内口服泼尼松775mg作为补充治疗。采用Laemili法并结合银盐浸渍，对治疗前、后非浓缩脑脊液和血清中的蛋白质进行电泳分离。对这些电泳图谱的分析显示olgG谱存在定性和定量变化。59%的病例发现定量变化，包括个别条带更明显的出现（8%的病例）或减弱（51%的病例）。29%的病例出现条带消除形式的定性变化，12%的病例出现新条带。作者得出结论，结果证实CY对缓慢进展型多发性硬化症患者的olgG具有强大的免疫抑制作用。
