Egilmez N K, Iwanuma Y, Bankert R B
Department of Molecular Immunology, Roswell Park Cancer Institute, Buffalo, New York 14263, USA.
Biochem Biophys Res Commun. 1996 Apr 5;221(1):169-73. doi: 10.1006/bbrc.1996.0564.
Five commonly used cationic liposome formulations were tested for their ability to deliver DNA to established subcutaneous human tumor xenografts in SCID mice. Liposomes were complexed with a mammalian expression plasmid containing the bacterial beta-galactosidase gene and delivered to tumors by direct injection. The optimal lipid to DNA ratios in vivo were markedly different than those observed in vitro for each liposome formulation. Tumor size at the time of inoculation also effected transfection efficiency significantly. Of the five liposome formulations tested, DC-Cholesterol was found to be superior to all others in vivo. Even under optimal conditions however, the efficiency of in vivo transfection was low in our system (approximately 0.3%). Implications of these results for in vivo gene therapy of tumors are discussed.
测试了五种常用的阳离子脂质体制剂将DNA递送至SCID小鼠体内已建立的皮下人肿瘤异种移植物的能力。脂质体与含有细菌β-半乳糖苷酶基因的哺乳动物表达质粒复合，并通过直接注射递送至肿瘤。每种脂质体制剂在体内的最佳脂质与DNA比例与体外观察到的明显不同。接种时的肿瘤大小也显著影响转染效率。在所测试的五种脂质体制剂中，发现二油酰基磷脂酰乙醇胺-胆固醇（DC-Cholesterol）在体内优于所有其他制剂。然而，即使在最佳条件下，我们系统中的体内转染效率也很低（约0.3%）。讨论了这些结果对肿瘤体内基因治疗的意义。
