Luo Y, Kessel D
Department of Pharmacology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA.
Biochem Biophys Res Commun. 1996 Apr 5;221(1):72-6. doi: 10.1006/bbrc.1996.0547.
DNA fragmentation and internucleosomal cleavage were rapidly initiated after lysosomal photodamage to murine leukemia cells, with apoptotic chromatin and DNA 'ladders' detected within 30 min after irradiation. Apoptosis was inhibited by concurrent exposure of cells to a 10 nM concentration of the serine-threonine phosphatase inhibitor calyculin A and promoted by a serine/threonine kinase inhibitor. These results indicate that a late stage in apoptosis requires serine/threonine dephosphorylation.
对鼠白血病细胞进行溶酶体光损伤后，DNA片段化和核小体间切割迅速启动，照射后30分钟内即可检测到凋亡染色质和DNA“梯形条带”。细胞同时暴露于10 nM浓度的丝氨酸 - 苏氨酸磷酸酶抑制剂花萼海绵诱癌素A时，凋亡受到抑制，而丝氨酸/苏氨酸激酶抑制剂则可促进凋亡。这些结果表明，凋亡的晚期阶段需要丝氨酸/苏氨酸去磷酸化。
