Mouyna I, Renard J L, Brygoo Y
Institut Pasteur, Paris, France.
Curr Genet. 1996 Jul 31;30(2):174-80. doi: 10.1007/s002940050117.
A worldwide collection, of 76 F. oxysporum f.sp. elaeidis isolates (Foe), and of 21 F. oxysporum isolates from the soil of several palm grove was analysed by RFLP. As a probe, we used a random DNA fragment (probe 46) from a genomic library of a Foe isolate. This probe contains two different types of sequence, one being repeated and dispersed in the genome "Palm", the other being a single-copy sequence. All F. oxysporum isolates from the palm-grove soils were non-pathogenic to oil palm. They all had a simple restriction pattern with one band homologous to the single-copy sequence of probe 46. All Foe isolates were pathogenic to oil palm and they all had complex patterns due to hybridization with "Palm". This repetitive sequence reveals that Foe isolates are distinct from the other F. oxysporum palm-grove soils isolates. The sequence can reliably discriminate pathogenic from non-pathogenic oil palm isolates. Based on DNA fingerprint similarities, Foe populations were divided into ten groups consisting of isolates with the same geographic origin. Isolates from Brazil and Ecuador were an exception to that rule as they had the same restriction pattern as a few isolates from the Ivory Coast, suggesting they may originated from Africa.
对来自世界各地的76株油棕尖孢镰刀菌（Foe）分离株以及从几个棕榈园土壤中分离得到的21株尖孢镰刀菌进行了限制性片段长度多态性（RFLP）分析。作为探针，我们使用了来自一株Foe分离株基因组文库的一个随机DNA片段（探针46）。该探针包含两种不同类型的序列，一种是在基因组“Palm”中重复且分散的序列，另一种是单拷贝序列。所有从棕榈园土壤中分离得到的尖孢镰刀菌对油棕均无致病性。它们都具有简单的限制性图谱，有一条与探针46的单拷贝序列同源的条带。所有Foe分离株对油棕都具有致病性，并且由于与“Palm”杂交，它们都具有复杂的图谱。这种重复序列表明Foe分离株与其他来自棕榈园土壤的尖孢镰刀菌分离株不同。该序列能够可靠地区分对油棕致病和不致病的分离株。基于DNA指纹相似性，Foe群体被分为十组，每组由具有相同地理来源的分离株组成。来自巴西和厄瓜多尔的分离株是个例外，因为它们与来自象牙海岸的一些分离株具有相同的限制性图谱，这表明它们可能起源于非洲。
