Edmunds R S, Simmons T A, Cox C F, Avery J K
J Oral Pathol. 1979 Apr;8(2):109-20. doi: 10.1111/j.1600-0714.1979.tb01630.x.
The interfaces and the relationships between collagen and oxytalan fibers were observed under light and electron microscopy. Guinea pig periodontal ligament was prepared for light and electron microscopy with perfusion using Peter's buffered formalin for light microscopy and GTA-S-collidine and OSO4 for electron microscopic studies. The tissue for light microscopy was stained with a modified Gomori's aldehyde fuchsin technique, in which pre-oxidization with potassium monopersulfate was carried out before staining so as to demonstrate the oxytalan fibers. EM tissues were routinely stained with lead citrate and uranyl acetate. Two different structural relationships were observed. First, the subcomponents of the collagen and oxytalan fiber types interweave with each other; and, second, some of these two-fiber subcomponents appear attached to each other. These relationships and the known orientation of oxytalan fibers as seen in the periodontal ligament provide insight as to the function of oxytalan fibers. The oxytalan fibers may provide increased structural integrity and increased distribution of forces over a wider area of the periodontal ligament. Because of their close relationship to blood and lymph vessels in the periodontal ligament, they may also help to stabilize these elements by the same structural relationships to collagen fibers.
在光学显微镜和电子显微镜下观察了胶原纤维和氧化弹力纤维之间的界面及关系。用彼得氏缓冲福尔马林灌注法制备豚鼠牙周韧带用于光学显微镜观察，用GTA-S-可力丁和四氧化锇制备用于电子显微镜研究的样本。光学显微镜观察的组织用改良的Gomori醛复红技术染色，染色前用过一硫酸钾进行预氧化以显示氧化弹力纤维。电子显微镜样本常规用柠檬酸铅和醋酸铀染色。观察到两种不同的结构关系。第一，胶原纤维和氧化弹力纤维的亚成分相互交织；第二，这两种纤维的一些亚成分似乎相互附着。这些关系以及在牙周韧带中观察到的氧化弹力纤维的已知取向为氧化弹力纤维的功能提供了线索。氧化弹力纤维可能会增强结构完整性，并在牙周韧带更广泛的区域增加力的分布。由于它们与牙周韧带中的血管和淋巴管关系密切，它们也可能通过与胶原纤维相同的结构关系来帮助稳定这些结构。
