Sculean A, Karring T, Theilade J, Lioubavina N
Department of Periodontology and Oral Gerontology, Royal Dental College, University of Aarhus, Denmark.
J Clin Periodontol. 1997 Dec;24(12):932-6. doi: 10.1111/j.1600-051x.1997.tb01214.x.
Although a number of studies have described the oxytalan fibers as being a natural component of the periodontal ligament, little information exists about the regenerative potential of these connective tissue fibers. The aim of the present study was to examine whether oxytalan fibers have the capacity to reform after regenerative periodontal therapy. Intrabony defects were produced surgically at the mesial aspects of teeth 37, 35, 45, 47 and at the distal aspects of teeth 11, 21, 31, 41 in one monkey (Macaca fascicularis). After 3 months, the defects were exposed using a full-thickness flap procedure. The root surfaces were debrided and subsequently PDGF-growth factors were placed in the defects. 4 of the 8 sites were covered with a bioresorbable membrane before closure of the wound. Post-surgically, antibiotics were given systemically for 1 week, and tooth cleaning was carried out 1x a week during the entire experimental period. After 5 months, the animal was sacrificed and the oral tissues were fixed by perfusion with 10% buffered formalin. Specimens containing the defects and surrounding tissues were dissected free and histological sections were cut in the mesio-distal direction, parallel to the long axes of the teeth. The sections were stained with hematoxylin and eosin or with the oxone-aldehyde-fuchsin-Halmi staining method and subsequently examined in the light and in the electron microscope. The results revealed that new oxytalan fibers oriented mainly in an apico-occlusal direction had developed in the regenerated periodontal ligament. Many of the newly-formed fibers were inserted into the new cementum, thus suggesting a strong relationship between this tissue and the oxytalan fibers. It is concluded that the regenerated periodontal ligament connective tissue formed after surgery contains oxytalan fibers similar to those present in the original tissue. These results demonstrate that oxytalan fibers develop de novo in the newly-formed periodontal ligament.
尽管许多研究已将弹力纤维描述为牙周韧带的天然组成部分，但关于这些结缔组织纤维的再生潜力的信息却很少。本研究的目的是检查弹力纤维在牙周再生治疗后是否有重新形成的能力。在一只猕猴（食蟹猴）的37、35、45、47号牙的近中面以及11、21、31、41号牙的远中面通过手术制造骨内缺损。3个月后，采用全厚瓣手术暴露缺损。对牙根表面进行清创，随后将血小板衍生生长因子（PDGF）-生长因子放置在缺损处。在伤口闭合前，8个部位中的4个用生物可吸收膜覆盖。术后，全身给予抗生素1周，在整个实验期间每周进行1次牙齿清洁。5个月后，处死动物，通过灌注10%缓冲福尔马林固定口腔组织。切取包含缺损和周围组织的标本，沿近远中方向、平行于牙齿长轴切成组织学切片。切片用苏木精和伊红染色或用臭氧-醛-品红-哈尔米染色法染色，随后在光学显微镜和电子显微镜下检查。结果显示，在再生的牙周韧带中已形成了主要沿根尖-咬合方向排列的新的弹力纤维。许多新形成的纤维插入到新的牙骨质中，这表明该组织与弹力纤维之间有密切关系。得出的结论是，手术后形成的再生牙周韧带结缔组织含有与原始组织中相似的弹力纤维。这些结果表明，弹力纤维在新形成的牙周韧带中重新形成。
