Basson M D, Turowski G, Emenaker N J
Department of Surgery, Yale University School of Medicine, New Haven, Connecticut 06520, USA. basson.marc_d+@west-haven.va.gov
Exp Cell Res. 1996 Jun 15;225(2):301-5. doi: 10.1006/excr.1996.0180.
Extracellular matrix regulation of intestinal epithelial differentiation may affect development, differentiation during migration to villus tips, healing, inflammatory bowel disease, and malignant transformation. Cell culture studies of intestinal epithelial biology may also depend on the matrix substrate used. We evaluated matrix effects on differentiation and proliferation in human intestinal Caco-2 epithelial cells, a model for intestinal epithelial differentiation. Proliferation, brush border enzyme specific activity, and spreading were compared in cells cultured on tissue culture plastic with interstitial collagen I and the basement membrane constituents collagen IV and laminin. Each matrix significantly increased alkaline phosphatase, dipeptidyl peptidase, lactase, sucrase-isomaltase, and cell spreading in comparison to plastic. However, the basement membrane proteins collagen IV and laminin further promoted all four brush border enzymes but inhibited spreading compared to collagen I. Proliferation was most rapid on type I collagen and slowest on laminin and tissue culture plastic. Basement membrane matrix proteins may promote intestinal epithelial differentiation and inhibit proliferation compared with interstitial collagen I.
细胞外基质对肠上皮分化的调节可能会影响发育、向绒毛顶端迁移过程中的分化、愈合、炎症性肠病以及恶性转化。肠上皮生物学的细胞培养研究也可能取决于所使用的基质底物。我们评估了基质对人肠Caco-2上皮细胞分化和增殖的影响，该细胞是肠上皮分化的模型。将在组织培养塑料上培养的细胞与间质胶原I以及基底膜成分胶原IV和层粘连蛋白进行比较，观察其增殖、刷状缘酶比活性和铺展情况。与塑料相比，每种基质均显著增加了碱性磷酸酶、二肽基肽酶、乳糖酶、蔗糖酶-异麦芽糖酶的活性以及细胞铺展。然而，与胶原I相比，基底膜蛋白胶原IV和层粘连蛋白进一步促进了所有四种刷状缘酶的活性，但抑制了细胞铺展。在I型胶原上增殖最快，在层粘连蛋白和组织培养塑料上最慢。与间质胶原I相比，基底膜基质蛋白可能促进肠上皮分化并抑制增殖。
