Goldmann W H, Ezzell R M
Surgery Research Unit, Harvard Medical School, Charlestown, Massachusetts, 02129, USA.
Exp Cell Res. 1996 Jul 10;226(1):234-7. doi: 10.1006/excr.1996.0223.
We have been studying mouse F9 embryonic carcinoma cells which contain no detectable vinculin protein (5.51 cells), and compared them with F9 wild-type cells. Employing atomic force microscopy, we probed the elastic properties of individual F9 wild-type and 5.51 cells by measuring the dynamic response of controlled loads of the cantilever tip. An elastic modulus (Young) of approximately 3.8 and approximately 2.5 kPa was calculated for wild-type and 5.51 cells, respectively. Using disc rheometry, we detected a marked change in shear of a 1000g pellet of approximately 55 x 10(6) cells between wild-type and 5.51 mutants. These differences are attributed to the loss of vinculin and altered cytoskeletal organization in these cells.
我们一直在研究不含可检测到的纽蛋白的小鼠F9胚胎癌细胞（5.51细胞），并将它们与F9野生型细胞进行比较。利用原子力显微镜，我们通过测量悬臂尖端受控负载的动态响应来探测单个F9野生型细胞和5.51细胞的弹性特性。分别计算出野生型细胞和5.51细胞的弹性模量（杨氏模量）约为3.8 kPa和约2.5 kPa。使用圆盘流变仪，我们检测到野生型细胞和5.51突变体细胞中约55×10⁶个细胞组成的1000g沉淀在剪切方面有显著变化。这些差异归因于这些细胞中纽蛋白的缺失和细胞骨架组织的改变。
