Boycott K M, Halley G R, Schlessinger D, Bech-Hansen N T
Department of Medical Genetics, University of Calgary, Calgary, Alberta, T2N 4N1, Canada.
Genomics. 1996 May 1;33(3):488-97. doi: 10.1006/geno.1996.0224.
A comprehensive physical contig of yeast artificial chromosomes (YACs) and cosmid clones between ZNF21 and DXS255 has been constructed, spanning 2 Mb within the region Xp11.23-p11.22. As a portion of the region was found to be particularly unstable in yeast, the integrity of the contig is dependent on additional information provided by the sequence-tagged site (STS) content of cosmid clones and DNA marker retention in conventional and radiation hybrids. The contig was formatted with 43 DNA markers, including 19 new STSs from YAC insert ends and an internal Alu-PCR product. The density of STSs across the contig ranges from one marker every 20 kb to one every 60 kb, with an average density of one marker every 50 kb. The relative order of previously known genes and expressed sequence tags in this region is predicted to be Xpter-ZNF21-DXS7465E (MG66)-DXS7927E (MG81)-WASP, DXS1011E, DXS7467E (MG21)-DXS- 7466E (MG44)-GATA1-DXS7469E (Xp664)-TFE3-SYP (DXS1007E)-Xcen. This contig extends the coverage in Xp11 and provides a framework for the future identification and mapping of new genes, as well as the resources for developing DNA sequencing templates.
已构建了酵母人工染色体（YAC）和黏粒克隆在ZNF21和DXS255之间的完整物理重叠群，跨越Xp11.23 - p11.22区域内的2 Mb。由于发现该区域的一部分在酵母中特别不稳定，重叠群的完整性依赖于黏粒克隆的序列标签位点（STS）含量以及常规和辐射杂种中DNA标记保留所提供的额外信息。该重叠群用43个DNA标记进行了格式化，包括来自YAC插入末端的19个新STS和一个内部Alu - PCR产物。整个重叠群中STS的密度范围从每20 kb一个标记到每60 kb一个标记，平均密度为每50 kb一个标记。预计该区域中先前已知基因和表达序列标签的相对顺序为Xpter - ZNF21 - DXS7465E（MG66） - DXS7927E（MG81） - WASP、DXS1011E、DXS7467E（MG21） - DXS - 7466E（MG44） - GATA1 - DXS7469E（Xp664） - TFE3 - SYP（DXS1007E） - Xcen。这个重叠群扩展了Xp11的覆盖范围，为未来新基因的鉴定和定位提供了框架，也为开发DNA测序模板提供了资源。
