在基因含量丰富的Xp11.23区域构建一个1.5兆碱基的细菌人工染色体重叠群。

Construction of a 1.5-Mb bacterial artificial chromosome contig in Xp11.23, a region of high gene content.

作者信息

Boycott K M, Zahorchak R J, Summer C G, Boycott N P, Kotak V, Russell C G, Bech-Hansen N T

机构信息

Department of Medical Genetics, Faculty of Medicine, University of Calgary, Alberta, Canada.

出版信息

Genomics. 1998 Mar 15;48(3):369-72. doi: 10.1006/geno.1997.5167.

DOI:10.1006/geno.1997.5167

PMID:9545643

Abstract

To generate sequence-ready templates for the gene-rich Xp11.23 region, we have constructed a 1.5-Mb bacterial artificial chromosome (BAC) contig spanning the interval between the DNA markers OATL1 and DXS255. The contig includes 28 BACs, ranging in size from 58 to 258 kb with an average size of 135 kb, which provide 2.5-fold coverage of the region. The BAC contig was constructed based entirely on the content of 40 DNA markers from a previously established YAC contig and 11 new markers developed from BAC-end DNA sequences, 4 of which were required to close gaps in the map. There was no evidence of rearrangement, instability, or chimerism in any of the BAC clones. The BAC cloning system appears to provide robust and total physical coverage of this gene-rich region with clones that are suitable for DNA sequencing.

摘要

为了生成富含基因的Xp11.23区域的序列就绪模板，我们构建了一个1.5兆碱基细菌人工染色体（BAC）重叠群，其跨越DNA标记OATL1和DXS255之间的区间。该重叠群包含28个BAC，大小从58千碱基到258千碱基不等，平均大小为135千碱基，提供了该区域2.5倍的覆盖率。该BAC重叠群完全基于先前建立的酵母人工染色体（YAC）重叠群中的40个DNA标记以及从BAC末端DNA序列开发的11个新标记构建而成，其中4个标记用于填补图谱中的缺口。在任何BAC克隆中均未发现重排、不稳定性或嵌合现象。BAC克隆系统似乎能用适合DNA测序的克隆为这个富含基因的区域提供强大且完整的物理覆盖。