Massung R F, Loparev V N, Knight J C, Totmenin A V, Chizhikov V E, Parsons J M, Safronov P F, Gutorov V V, Shchelkunov S N, Esposito J J
Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, Atlanta, Georgia 30333, USA.
Virology. 1996 Jul 15;221(2):291-300. doi: 10.1006/viro.1996.0378.
Genome DNA terminal region sequences were determined for a Brazilian alastrim variola minor virus strain Garcia-1966 that was associated with an 0.8% case-fatality rate and African smallpox strains Congo-1970 and Somalia-1977 associated with variola major (9.6%) and minor (0.4%) mortality rates, respectively. A base sequence identity of > or = 98.8% was determined after aligning 30 kb of the left- or right-end region sequences with cognate sequences previously determined for Asian variola major strains India-1967 (31% death rate) and Bangladesh-1975 (18.5% death rate). The deduced amino acid sequences of putative proteins of > or = 65 amino acids also showed relatively high identity, although the Asian and African viruses were clearly more related to each other than to alastrim virus. Alastrim virus contained only 10 of 70 proteins that were 100% identical to homologs in Asian strains, and 7 alastrim-specific proteins were noted.
测定了一株巴西类天花痘苗病毒小株Garcia - 1966的基因组DNA末端区域序列，该毒株的病死率为0.8%，还测定了非洲天花毒株Congo - 1970和Somalia - 1977的基因组DNA末端区域序列，这两种毒株分别与大天花（病死率9.6%）和小天花（病死率0.4%）相关。将左端或右端区域30 kb的序列与先前为亚洲大天花毒株India - 1967（病死率31%）和Bangladesh - 1975（病死率18.5%）测定的同源序列进行比对后，确定碱基序列同一性≥98.8%。推导的氨基酸序列中长度≥65个氨基酸的假定蛋白质也显示出相对较高的同一性，尽管亚洲和非洲病毒彼此之间的关系明显比与类天花病毒的关系更为密切。类天花病毒在70种蛋白质中只有10种与亚洲毒株中的同源物100%相同，并且发现了7种类天花病毒特异性蛋白质。
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