Massung R F, Liu L I, Qi J, Knight J C, Yuran T E, Kerlavage A R, Parsons J M, Venter J C, Esposito J J
Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333.
Virology. 1994 Jun;201(2):215-40. doi: 10.1006/viro.1994.1288.
We analyzed the 186,102 base pairs (bp) that constitute the entire DNA genome of a highly virulent variola virus isolated from Bangladesh in 1975. The linear, double-stranded molecule has relatively small (725 bp) inverted terminal repeat (ITR) sequences containing three 69-bp direct repeat elements, a 54-bp partial repeat element, and a 105-base telomeric end-loop that can be maximally base-paired to contain 17 mismatches. Proximal to the right-end ITR sequences are another seven 69-bp elements and a 53- and a 27-bp partial element. Sequence analysis showed 187 closely spaced open reading frames specifying putative major proteins containing > or = 65 amino acids. Most of the virus proteins correspond to proteins in current databases, including 150 proteins that have > 90% identity to major gene products encoded by vaccinia virus, the smallpox vaccine. Variola virus has a group of proteins that are truncated compared with vaccinia virus counterparts and a smaller group of proteins that are elongated. The terminal regions encode several novel proteins and variants of other poxvirus proteins that potentially augment variola virus transmissibility and virulence for its only natural host, humans.
我们分析了1975年从孟加拉国分离出的一种高致病性天花病毒的整个DNA基因组，其由186,102个碱基对（bp）组成。该线性双链分子具有相对较小（725 bp）的反向末端重复（ITR）序列，其中包含三个69 bp的直接重复元件、一个54 bp的部分重复元件以及一个105个碱基的端粒末端环，该末端环可通过最大程度碱基配对包含17个错配。在右端ITR序列近端还有另外七个69 bp元件以及一个53 bp和一个27 bp的部分元件。序列分析显示有187个紧密间隔的开放阅读框，它们指定了推定的主要蛋白质，这些蛋白质包含≥65个氨基酸。大多数病毒蛋白与当前数据库中的蛋白质相对应，其中包括150种与天花疫苗牛痘病毒编码的主要基因产物具有> 90％同一性的蛋白质。天花病毒有一组与牛痘病毒对应蛋白相比被截短的蛋白质，以及一小群被延长的蛋白质。末端区域编码几种新蛋白质以及其他痘病毒蛋白质的变体，这些变体可能会增强天花病毒对其唯一天然宿主人类的传播性和毒力。
