通过在琼脂糖偶联的氨基苯基磷酰尿苷-2'（3'）-磷酸上进行亲和层析从脱氧核糖核酸酶中去除核糖核酸酶活性。
Removal of RNase activity from DNase by affinity chromatography on agarose coupled aminophenylphosphoryl-uridine-2' (3')-phosphate.
作者信息
Maxwell I H, Maxwell F, Hahn W E
出版信息
Nucleic Acids Res. 1977 Jan;4(1):241-6. doi: 10.1093/nar/4.1.241.
Abstract
Severe degradation of high molecular weight RNA was shown to occur during incubation with commercially purified DNase. Most of the RNase activity could be removed by passage of the DNase through a column of agarose-coupled amino phenylphosphoryl-uridine-2' (3')-phosphate. Incubation with the treated DNase caused only minimal alteration of the sedimentation pattern of high molecular weight nuclear RNA, determined under partially denturing conditions. No impairment of DNase activity was detected.
摘要
结果表明，在与商业纯化的脱氧核糖核酸酶（DNase）孵育过程中，高分子量RNA会发生严重降解。通过使DNase通过琼脂糖偶联的氨苯基磷酰尿苷-2'（3'）-磷酸柱，可以去除大部分核糖核酸酶（RNase）活性。在部分变性条件下测定，与经处理的DNase孵育仅导致高分子量核RNA沉降模式的微小改变。未检测到DNase活性受损。
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