从哺乳动物组织中分离脱氧核糖核酸。
Isolation of deoxyribonucleic acid from mammalian tissues.
作者信息
Kirby K S, Cook E A
出版信息
Biochem J. 1967 Jul;104(1):254-7. doi: 10.1042/bj1040254.
Abstract
- DNA has been isolated from different mammalian tissues. The DNA preparations were free from RNA, protein and polysaccharides and have a similar range of sedimentation coefficients (approx. 24s). 2. Protein was removed by a two-stage extraction with a phenol-cresol mixture by using a detergent with 4-aminosalicylate in the first stage and sodium chloride in the second. 3. Polysaccharides remained in solution when DNA was precipitated with 2-butoxyethanol in the presence of 0.5m-sodium chloride and 1.5m-sodium benzoate. 4. Ribosomal RNA was removed by precipitation in the presence of 3m-sodium chloride at 0 degrees , when DNA remained soluble.
摘要
- 已从不同的哺乳动物组织中分离出DNA。这些DNA制剂不含RNA、蛋白质和多糖，且沉降系数范围相似（约24s）。2. 通过用苯酚 - 甲酚混合物进行两阶段提取来去除蛋白质，第一阶段使用含有4 - 氨基水杨酸盐的去污剂，第二阶段使用氯化钠。3. 当在0.5m氯化钠和1.5m苯甲酸钠存在下用2 - 丁氧基乙醇沉淀DNA时，多糖仍留在溶液中。4. 在0摄氏度、3m氯化钠存在下进行沉淀可去除核糖体RNA，此时DNA仍可溶。
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