溶液中游离的卤虫（盐水虾）核糖体RNA的大小和构象。
The size and conformation of Artemia (brine-shrimp) ribosomal RNA free in solution.
作者信息
Donceel K, Nieuwenhuysen P, Clauwaert J
出版信息
Biochem J. 1982 Sep 1;205(3):495-501. doi: 10.1042/bj2050495.
The RNA was isolated from the large ribosomal subunits of the brine shrimp Artemia, and its conformation free in solution was studied by determining its sedimentation and diffusion coefficients. A comparison was made of the hydrodynamic radius of the ribosomal subunit and its isolated RNA in various buffers. The conformation of the rRNA free in solution is more extended than when it is incorporated in the ribosome. This is not only the case when the rRNA solution lacks bivalent and polyvalent cations, but even in the presence of Mg2+ and spermidine, which cause a tightening of RNA. Thus the ribosomal proteins should induce a further tightening of the rRNA during the assembly of the ribosome. In the discussion, the reported data on Escherichia coli rRNA species are presented in such a way that large discrepancies between various studied are revealed, and that they can be compared with the data reported here on the larger rRNA of an eukaryote.
从卤虫的大核糖体亚基中分离出RNA，并通过测定其沉降系数和扩散系数来研究其在溶液中的自由构象。对核糖体亚基及其分离出的RNA在各种缓冲液中的流体动力学半径进行了比较。溶液中自由存在的rRNA的构象比其掺入核糖体时更为伸展。不仅当rRNA溶液缺乏二价和多价阳离子时如此，甚至在存在Mg2+和亚精胺（它们会使RNA收紧）的情况下也是如此。因此，核糖体蛋白在核糖体组装过程中应会使rRNA进一步收紧。在讨论中，以揭示不同研究之间的巨大差异并能将其与这里报道的真核生物较大rRNA的数据进行比较的方式呈现了关于大肠杆菌rRNA种类的已报道数据。
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