DNase II消化后大鼠肝脏染色质组分的聚赖氨酸滴定

Polylysine titration of rat liver chromatin fractions after DNase II digestion.

作者信息

Vauhkonen M, Hemminki K

出版信息

Nucleic Acids Res. 1977 Apr;4(4):791-9. doi: 10.1093/nar/4.4.791.

DOI:10.1093/nar/4.4.791

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342484/

Abstract

The concentration of free phosphate groups is measured in rat liver chromatin after DNase II digestion using polylysine titration. The unsheared chromatin completely precipitates at lysine/DNA phosphate ratios of 0.5 to 0.6. Digestion of the chromatin reduces the lysine/DNA phosphate ratio of complete precipitation by about 0.2 units suggesting the removal of free phosphate groups. The two chromatin fractions: MgC12 insoluble (template-inactive) and Mg12 soluble (template-active) chromatins precipitate at about the same lysine/DNA phosphate ratio. Some 15% of the MgC12 soluble chromatin remains in solution at any polylysine concentration. The removal of histone H 1 FROM THE MgC12 insoluble chromatin increases the lysine/DNA phosphate ratio by about 0.2 units suggesting that 20% of the DNA phosphate groups in nucleosomes are masked by histone H 1.

摘要

使用聚赖氨酸滴定法在DNase II消化后的大鼠肝脏染色质中测量游离磷酸基团的浓度。未剪切的染色质在赖氨酸/DNA磷酸比为0.5至0.6时完全沉淀。染色质的消化使完全沉淀的赖氨酸/DNA磷酸比降低约0.2个单位，表明游离磷酸基团被去除。两种染色质组分：MgC12不溶性（模板无活性）和Mg12可溶性（模板有活性）染色质在大约相同的赖氨酸/DNA磷酸比下沉淀。在任何聚赖氨酸浓度下，约15%的MgC12可溶性染色质仍留在溶液中。从MgC12不溶性染色质中去除组蛋白H1会使赖氨酸/DNA磷酸比增加约0.2个单位，这表明核小体中20%的DNA磷酸基团被组蛋白H1掩盖。

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