非洲爪蟾卵巢中的组蛋白mRNA：H4信使核糖核酸的鉴定。
Histone mRNA in Xenopus laevis ovaries: identification of the H4 messenger.
作者信息
Destrée O H, Haenni A L, Birnstiel M L
出版信息
Nucleic Acids Res. 1977 Apr;4(4):801-11. doi: 10.1093/nar/4.4.801.
Abstract
RNA extracted from ovaries of adult Xenopus laevis and introduced into a wheat germ cell-free system, directs the synthesis of polypeptides co-electrophoresing with all five histones. By frationation of total ovary RNA through Sephadex G-200 and polyacrylamide gel, RNA was isolated that is translated into histones 4 (H4) only. In addition, this RNA labelled in vitro with [I125] hybridizes to a restriction fragment of cloned sea urchin DNA known to contain the H4 gene.
摘要
从成年非洲爪蟾卵巢中提取的RNA，被引入无细胞小麦胚芽系统后，可指导与所有五种组蛋白共电泳的多肽的合成。通过用葡聚糖凝胶G - 200和聚丙烯酰胺凝胶对总卵巢RNA进行分级分离，分离出了仅被翻译成组蛋白4（H4）的RNA。此外，这种在体外用[I125]标记的RNA与已知含有H4基因的克隆海胆DNA的一个限制性片段杂交。
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