非洲爪蟾的H3和H4组蛋白cDNA序列：H4基因的序列比较
H3 and H4 histone cDNA sequences from Xenopus: a sequence comparison of H4 genes.
作者信息
Turner P C, Woodland H R
出版信息
Nucleic Acids Res. 1982 Jun 25;10(12):3769-80. doi: 10.1093/nar/10.12.3769.
Abstract
Ovarian poly (A) + RNA from Xenopus laevis and Xenopus borealis was used to construct two cDNA libraries which were screened for histone sequences. cDNA clones to H4 mRNA were obtained from both species and an H3 cDNA clone from Xenopus laevis. The complete DNA sequences of these clones have been determined and are presented. These new sequences are compared with other H3 and H4 DNA sequences both in the coding and 3' noncoding regions. We find that there is considerable non-random codon usage in ten H4 genes. In addition there are some sequence similarities in the 3' noncoding regions of H3 and H4 genes.
摘要
来自非洲爪蟾和北美爪蟾的卵巢多聚腺苷酸（poly (A)）+ RNA被用于构建两个cDNA文库，这些文库针对组蛋白序列进行了筛选。从这两个物种中均获得了H4 mRNA的cDNA克隆，以及一个来自非洲爪蟾的H3 cDNA克隆。已确定并展示了这些克隆的完整DNA序列。将这些新序列在编码区和3'非编码区与其他H3和H4 DNA序列进行了比较。我们发现在十个H4基因中存在相当多的非随机密码子使用情况。此外，H3和H4基因的3'非编码区存在一些序列相似性。
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