核糖核酸酶A-寡核苷酸相互作用的圆二色光谱研究
CD studies on ribonuclease A - oligonucleotides interactions.
作者信息
White M D, Keren-Zur M, Lapidot Y
出版信息
Nucleic Acids Res. 1977 Apr;4(4):843-52. doi: 10.1093/nar/4.4.843.
Abstract
The interaction of ApU, Aps4U, Aps4Up, ApAps4Up and Gps4U with RNase A was studied by CD difference spectroscopy. The use of 4-thiouridine (s4U) containing oligonucleotides enables to distinguish between the interaction of the different components of the ligand with the enzyme. The mode of binding of the oligonucleotides to the enzyme is described. From this mode of binding it is explained why Aps4U, for example, inhibits RNase A, while s4UpA serves as a substrate.
摘要
通过圆二色差光谱研究了ApU、Aps4U、Aps4Up、ApAps4Up和Gps4U与核糖核酸酶A的相互作用。使用含4-硫尿苷（s4U）的寡核苷酸能够区分配体不同组分与该酶的相互作用。描述了寡核苷酸与该酶的结合模式。从这种结合模式解释了为什么例如Aps4U会抑制核糖核酸酶A，而s4UpA作为底物。
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