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核糖核酸酶A-寡核苷酸相互作用的圆二色光谱研究

CD studies on ribonuclease A - oligonucleotides interactions.

作者信息

White M D, Keren-Zur M, Lapidot Y

出版信息

Nucleic Acids Res. 1977 Apr;4(4):843-52. doi: 10.1093/nar/4.4.843.

DOI:10.1093/nar/4.4.843
PMID:866194
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342488/
Abstract

The interaction of ApU, Aps4U, Aps4Up, ApAps4Up and Gps4U with RNase A was studied by CD difference spectroscopy. The use of 4-thiouridine (s4U) containing oligonucleotides enables to distinguish between the interaction of the different components of the ligand with the enzyme. The mode of binding of the oligonucleotides to the enzyme is described. From this mode of binding it is explained why Aps4U, for example, inhibits RNase A, while s4UpA serves as a substrate.

摘要

通过圆二色差光谱研究了ApU、Aps4U、Aps4Up、ApAps4Up和Gps4U与核糖核酸酶A的相互作用。使用含4-硫尿苷（s4U）的寡核苷酸能够区分配体不同组分与该酶的相互作用。描述了寡核苷酸与该酶的结合模式。从这种结合模式解释了为什么例如Aps4U会抑制核糖核酸酶A，而s4UpA作为底物。

相似文献

1
CD studies on ribonuclease A - oligonucleotides interactions.核糖核酸酶A-寡核苷酸相互作用的圆二色光谱研究
Nucleic Acids Res. 1977 Apr;4(4):843-52. doi: 10.1093/nar/4.4.843.
2
[Substrate specificity of RNase from Actinomyces sp., strain 236, producing mono- and oligonucleotides with 5'-phosphate end groups].
Biokhimiia. 1978 Jan;43(1):40-9.
3
Inhibition of pancreatic ribonuclease by 2'-5' and 3'-5' oligonucleotides.2'-5'和3'-5'寡核苷酸对胰腺核糖核酸酶的抑制作用
Nucleic Acids Res. 1977 Sep;4(9):3029-38. doi: 10.1093/nar/4.9.3029.
4
Stepwise synthesis of oligonucleotides. Synthesis of trinucleoside diphosphates catalyzed by a nonspecific ribonuclease from Penicillium brevicompactum.寡核苷酸的逐步合成。短密青霉非特异性核糖核酸酶催化三核苷二磷酸的合成。
Mol Biol. 1974 May;7(6):649-54.
5
Recruitment of divalent metal ions by incorporation of 4-thio-2'-deoxythymidine or 4-thio-2'-deoxyuridine into DNA.通过将4-硫代-2'-脱氧胸苷或4-硫代-2'-脱氧尿苷掺入DNA来招募二价金属离子。
J Biol Inorg Chem. 2004 Jun;9(4):462-70. doi: 10.1007/s00775-004-0545-0. Epub 2004 Apr 30.
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Interactions between oligonucleotides having a left-handed helical structure and ethidium bromide.具有左手螺旋结构的寡核苷酸与溴化乙锭之间的相互作用。
Nucleic Acids Symp Ser. 1980(8):s147-50.
7
Chemical modification of ribonuclease T1 with ozone.用臭氧对核糖核酸酶T1进行化学修饰。
J Biochem. 1978 Mar;83(3):771-81. doi: 10.1093/oxfordjournals.jbchem.a131971.
8
Binding of 8-bromoguanylic acid to ribonuclease T1 as studied by absorption and circular dichroism spectroscopy.通过吸收光谱和圆二色光谱研究8-溴鸟苷酸与核糖核酸酶T1的结合。
Biochem Biophys Res Commun. 1983 Jul 18;114(1):88-92. doi: 10.1016/0006-291x(83)91597-8.
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A novel fluorogenic substrate for ribonucleases. Synthesis and enzymatic characterization.一种用于核糖核酸酶的新型荧光底物。合成与酶学特性研究。
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10
Subsites and catalytic mechanism of ribonuclease T: kinetic studies using GpC and GpU as substrates.
Arch Biochem Biophys. 1976 Aug;175(2):558-64. doi: 10.1016/0003-9861(76)90545-2.

本文引用的文献

1
The properties of bovine pancreatic ribonuclease in ethylene glycol solution.牛胰核糖核酸酶在乙二醇溶液中的性质
Biochemistry. 1962 Mar;1:305-17. doi: 10.1021/bi00908a018.
2
Research on pancreatic ribonuclease. I. The inhibition of cyclic phospho-diesterase activity of bovine pancreatic ribonuclease by several substrate analogues.胰腺核糖核酸酶的研究。I. 几种底物类似物对牛胰腺核糖核酸酶环磷酸二酯酶活性的抑制作用。
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3
Optical rotatory dispersion and circular dichroism of sulfur-containing nucleosides and nucleotide and of the ribonuclease-thionucleotide complex.含硫核苷、核苷酸以及核糖核酸酶 - 硫代核苷酸复合物的旋光色散和圆二色性
Biochim Biophys Acta. 1969 Mar 18;179(1):1-9. doi: 10.1016/0005-2787(69)90115-4.
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Interaction between bovine pancreatic ribonuclease and 4-thiouridylic acid.
J Biochem. 1968 Aug;64(2):161-5. doi: 10.1093/oxfordjournals.jbchem.a128875.
5
Do the exposed tyrosine residues of ribonuclease A interact with nucleotides?核糖核酸酶A中暴露的酪氨酸残基会与核苷酸相互作用吗？
J Biochem. 1967 Aug;62(2):282-4. doi: 10.1093/oxfordjournals.jbchem.a128662.
6
Conformation of cytidine group in the complex of pancreatic ribonuclease A and cytidine-2'-phosphate.胰腺核糖核酸酶A与胞苷-2'-磷酸复合物中胞苷基团的构象
J Biochem. 1971 Jul;70(1):193-5. doi: 10.1093/oxfordjournals.jbchem.a129620.
7
Interaction of uridine-2'(3'),5'-diphosphate withibonuclease A and carboxymethylribonuclease A.尿苷-2'(3'),5'-二磷酸与核糖核酸酶A和羧甲基核糖核酸酶A的相互作用。
J Biochem. 1969 Sep;66(3):415-8. doi: 10.1093/oxfordjournals.jbchem.a129162.
8
On the binding of chitin oligosaccharides to lysozyme.关于几丁质寡糖与溶菌酶的结合
Proc Natl Acad Sci U S A. 1966 Jul;56(1):26-30. doi: 10.1073/pnas.56.1.26.
9
Conformational changes in some ribonuclease-inhibitor complexes.某些核糖核酸酶抑制剂复合物中的构象变化。
Biochim Biophys Acta. 1971 Oct;251(1):126-31. doi: 10.1016/0005-2795(71)90070-5.
10
Magnesium-induced conformational change in transfer ribonucleic acid as measured by circular dichroism.通过圆二色性测量镁诱导的转移核糖核酸构象变化。
Biochemistry. 1971 Jun 8;10(12):2216-22. doi: 10.1021/bi00788a005.