Koshcheenko K A, Baklashova T G, Kozlovskiĭ A G, Arinbasarov M U, Skriabin G K
Prikl Biokhim Mikrobiol. 1977 Mar-Apr;13(2):248-54.
Physiological and biochemical properties of the culture of Aspergillus niger IBPM F 12 carrying out hydroxylation IAA in the 4-, 5-, 6-positions of the indole nucleus were studied. The optimal composition of the medium for the cultivation was established. The transformation was performed by the washed fungal mycelium taken in the middle of the growth log-phase at a substrate concentration of I g/l. The correlation between the hydroxylase activity and pH, temperaure and biomass quantity was shown. The method of isolating 4-, 5- and 6-hydroxyindolyl-3-acetic acids in preparative amounts was developed.
研究了黑曲霉IBPM F 12培养物的生理生化特性，该培养物可在吲哚核的4、5、6位进行吲哚-3-乙酸（IAA）的羟基化反应。确定了用于培养的培养基的最佳组成。转化反应由处于生长对数中期的洗涤过的真菌菌丝体进行，底物浓度为1 g/l。显示了羟化酶活性与pH、温度和生物量之间的相关性。开发了以制备量分离4-、5-和6-羟基吲哚-3-乙酸的方法。
