Mayer T, Touchot N, Elazar Z
Department for Experimental Tumor Biology, University of Münster, Badestrasse 9, 48149 Münster, Germany.
J Biol Chem. 1996 Jul 5;271(27):16097-103. doi: 10.1074/jbc.271.27.16097.
The small GTP-binding protein Rab6, a member of the Ras superfamily, is localized on the membranes of the Golgi apparatus and the trans Golgi network. Recent studies revealed that the Rab6 protein might be involved in the transit of proteins through the Golgi complex. In this report we demonstrate the essential function of the Rab6 protein in a distinct step of reconstituted Golgi transport. Polyclonal antibodies and Fab fragments directed against the C-terminal part of the Rab6 protein inhibit transport between the cis and the medial Golgi cisternae. Inhibition also occurred when a trans-dominant mutant form of the Rab6 protein (N126I) was added to the reconstituted transport. Furthermore, Rab6 antibodies inhibit uncoupled fusion of Golgi membranes. From these data we conclude that Rab6 is involved in a process related to membrane fusion at the cisternal membranes of the Golgi apparatus and therefore is needed for the consumption of Golgi-derived vesicles by their target membranes.
小GTP结合蛋白Rab6是Ras超家族的成员之一，定位于高尔基体和反式高尔基体网络的膜上。最近的研究表明，Rab6蛋白可能参与蛋白质通过高尔基体复合体的转运过程。在本报告中，我们证明了Rab6蛋白在重构的高尔基体运输的一个特定步骤中的重要功能。针对Rab6蛋白C末端部分的多克隆抗体和Fab片段可抑制顺式和中间高尔基体潴泡之间的运输。当将Rab6蛋白的反式显性突变形式（N126I）添加到重构运输体系中时，也会出现抑制现象。此外，Rab6抗体可抑制高尔基体膜的非偶联融合。从这些数据我们得出结论，Rab6参与了与高尔基体潴泡膜融合相关的过程，因此其靶膜消耗源自高尔基体的囊泡需要Rab6。
