Dietz K J, Arbinger B
Julius-von-Sachs-Institut für Biowissenschaften, Julius-Maximilians-Universität Würzburg, Germany.
Biochim Biophys Acta. 1996 Jun 11;1281(2):134-8. doi: 10.1016/0005-2736(96)00044-2.
A cDNA coding for subunit E of the vacuolar H(+)-ATPase was cloned from Mesembryanthemum crystallinum, a plant which switches from C3-photosynthesis to Crassulacean acid metabolism under saline growth conditions. Sequence homology between the three subunit E-polypeptides of different higher plant species varied between 77.6 and 73.3%; peptide length was between 226 and 230 amino acid residues, 43 of which are invariant in all seven subunit E-polypeptides known so far from animals, fungi and plants. The deduced relative molecular mass of subunit E in Mesembryanthemum crystallinum is 26162 Da. Subunit E is present both in C3- and CAM-plants. mRNA levels increased severalfold in leaves of CAM-induced plants. This was accompanied by a less pronounced increase in subunit E protein. Obviously, expression is stimulated under conditions of increased requirement for tonoplast H(+)-pumping activity.
从冰叶日中花中克隆出了编码液泡H(+)-ATP酶E亚基的cDNA，冰叶日中花是一种在盐渍生长条件下从C3光合作用转变为景天酸代谢的植物。不同高等植物物种的三种E亚基多肽之间的序列同源性在77.6%至73.3%之间；肽长度在226至230个氨基酸残基之间，其中43个在目前已知的来自动物、真菌和植物的所有七个E亚基多肽中是不变的。冰叶日中花中E亚基的推导相对分子质量为26162 Da。E亚基在C3植物和景天酸代谢植物中均有存在。在诱导景天酸代谢的植物叶片中，mRNA水平增加了几倍。与此同时，E亚基蛋白的增加不太明显。显然，在液泡膜H(+)泵浦活性需求增加的条件下，表达受到刺激。
