Kang J S, Choi C M, Yun I
Department of Oral Biochemistry and Molecular Biology, College of Dentistry and Research Institute for Oral Biotechnology, Pusan National University, South Korea.
Biochim Biophys Acta. 1996 Jun 11;1281(2):157-63. doi: 10.1016/0005-2736(95)00301-0.
Intramolecular excimerization of Py-3-Py and fluorescence polarization of DPH were used to evaluate effects of ethanol on the rate and range of the lateral mobility and the range of the rotational mobility of bulk bilayer structures of the Sp2/0-PMV. In a concentration-dependent manner, ethanol increased the rate and range of the lateral mobility and the range of the rotational mobility of bulk bilayer structures of Sp2/0-PMV. Selective quenching of DPH by trinitrophenyl groups was utilized to examine the range of transbilayer asymmetric rotational mobility of the Sp2/0-PVM. The anisotropy (r), limiting anisotropy (r(infinity)) and order parameter (S) of DPH in the inner monolayer were 0.022, 0.029 and 0.063, respectively, greater than calculated for the outer monolayer of the Sp2/0-PMV. Selective quenching of DPH by trinitrophenyl groups was also used to examine the transbilayer asymmetric effects of ethanol on the range of the rotational mobility of the Sp2/0-PMV. Ethanol had a greater increasing effect on the range of the rotational mobility of the outer monolayer as compared to the inner monolayer of the Sp2/0-PMV. It has been proven that ethanol exhibits a selective rather than nonselective fluidizing effect within the transbilayer domains of the Sp2/0-PMV.
利用Py-3-Py的分子内激基缔合物形成和DPH的荧光偏振来评估乙醇对Sp2/0-PMV本体双层结构横向迁移率和范围以及旋转迁移率范围的影响。乙醇以浓度依赖的方式增加了Sp2/0-PMV本体双层结构的横向迁移率和范围以及旋转迁移率范围。利用三硝基苯基对DPH的选择性猝灭来研究Sp2/0-PVM跨双层不对称旋转迁移率的范围。DPH在内侧单层中的各向异性（r）、极限各向异性（r(∞)）和序参数（S）分别为0.022、0.029和0.063，大于Sp2/0-PMV外侧单层的计算值。三硝基苯基对DPH的选择性猝灭也用于研究乙醇对Sp2/0-PMV旋转迁移率范围的跨双层不对称影响。与Sp2/0-PMV的内侧单层相比，乙醇对外侧单层旋转迁移率范围的增加作用更大。已证明乙醇在Sp2/0-PMV的跨双层区域内表现出选择性而非非选择性的流化作用。
