Yun I, Lee S H, Kang J S
Department of Dental Pharmacology and Biophysics, College of Dentistry, Pusan National University, Korea.
J Membr Biol. 1994 Mar;138(3):221-7. doi: 10.1007/BF00232794.
Intramolecular excimer formation of 1,3-di(1-pyrenyl)propane (Py-3-Py) and fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene (DPH) were used to evaluate the effect of ethanol on the rate and range of the lateral mobility and the range of the rotational mobility of bulk bilayer structures of the plasma membrane vesicles (ATCC-PMV) isolated from cultured hybridoma cells (ATCC TIB 216). In a concentration-dependent manner, ethanol increased the excimer to monomer fluorescence intensity ratio (I'/I) of Py-3-Py in the ATCC-PMV and decreased the anisotropy (r), limiting anisotropy (r infinity) and order parameter (S) of DPH in the ATCC-PMV. This indicates that ethanol increased both the lateral and rotational mobility of the probes in the ATCC-PMV. Selective quenching of DPH by trinitrophenyl groups was utilized to examine the range of transbilayer asymmetric rotational diffusion of the ATCC-PMV. The anisotropy (r), limiting anisotropy (r infinity) and order parameter (S) of DPH in the inner monolayer were 0.024, 0.032, and 0.069, respectively, greater than calculated for the outer monolayer of the ATCC-PMV. Selective quenching of DPH by trinitrophenyl groups was also used to examine the transbilayer asymmetric effects of ethanol on the range of the rotational mobility of the ATCC-PMV. Ethanol had a greater increasing effect on the range of the rotational mobility of the outer monolayer as compared to the inner monolayer of the ATCC-PMV. It has been proven that ethanol exhibits a selective rather than nonselective fluidizing effect within the transbilayer domains of the ATCC-PMV.
利用1,3 - 二（1 - 芘基）丙烷（Py - 3 - Py）的分子内激基缔合物形成以及1,6 - 二苯基 - 1,3,5 - 己三烯（DPH）的荧光偏振来评估乙醇对从培养的杂交瘤细胞（ATCC TIB 216）分离的质膜囊泡（ATCC - PMV）的整体双层结构的横向迁移率和范围以及旋转迁移率范围的影响。乙醇以浓度依赖的方式增加了ATCC - PMV中Py - 3 - Py的激基缔合物与单体荧光强度比（I'/I），并降低了ATCC - PMV中DPH的各向异性（r）、极限各向异性（r∞）和序参数（S）。这表明乙醇增加了ATCC - PMV中探针的横向和旋转迁移率。利用三硝基苯基基团对DPH的选择性猝灭来研究ATCC - PMV的跨双层不对称旋转扩散范围。ATCC - PMV内单层中DPH的各向异性（r）、极限各向异性（r∞）和序参数（S）分别比外单层计算值大0.024、0.032和0.069。还利用三硝基苯基基团对DPH的选择性猝灭来研究乙醇对ATCC - PMV旋转迁移率范围的跨双层不对称影响。与ATCC - PMV的内单层相比，乙醇对ATCC - PMV外单层的旋转迁移率范围具有更大的增加作用。已证明乙醇在ATCC - PMV的跨双层区域内表现出选择性而非非选择性的流化作用。
