大豆疫霉无毒基因、随机扩增多态性DNA（RAPD）和限制性片段长度多态性（RFLP）标记用于构建详细的遗传连锁图谱。

Phytophthora sojae avirulence genes, RAPD, and RFLP markers used to construct a detailed genetic linkage map.

作者信息

Whisson S C, Drenth A, Maclean D J, Irwin J A

机构信息

Cooperative Research Centre for Tropical Plant Pathology, University of Queensland, Brisbane, Australia.

出版信息

Mol Plant Microbe Interact. 1995 Nov-Dec;8(6):988-95. doi: 10.1094/mpmi-8-0988.

DOI:10.1094/mpmi-8-0988

PMID:8664507

Abstract

Two crosses between different races of Phytophthora sojae were established using one race as a common parent in both crosses. F2 populations comprising over 200 individuals were generated for each cross. A subset of 53 F2 individuals from each cross was selected at random for genetic analysis of virulence/avirulence and molecular markers, and finally the construction of a detailed genetic linkage map. The linkage map developed for P. sojae is based on a total of 257 markers (22 RFLP, 228 RAPD, and 7 avirulence genes). The linkage map comprises 10 major and 12 minor linkage groups covering a total of 830.5 cM. Close linkage was observed between Avr4 and Avr6 (0.0 cM), Avr1b and Avr1k (0.0 cM), and Avr3a and Avr5 (4.6 cM). Coupling phase linkage of RFLP and RAPD markers to all seven avirulence genes was identified at the minimum and maximum distances of 0.0 and 14.7 cM, respectively.

摘要

以一种小种作为两个杂交组合的共同亲本，构建了两个不同小种的大豆疫霉杂交组合。每个杂交组合均产生了包含200多个个体的F2群体。从每个杂交组合中随机选取53个F2个体，用于毒性/无毒性的遗传分析和分子标记，最终构建详细的遗传连锁图谱。为大豆疫霉构建的连锁图谱基于总共257个标记（22个RFLP、228个RAPD和7个无毒性基因）。该连锁图谱包含10个主要连锁群和12个次要连锁群，总共覆盖830.5 cM。观察到Avr4和Avr6之间紧密连锁（0.0 cM）、Avr1b和Avr1k之间紧密连锁（0.0 cM）以及Avr3a和Avr5之间紧密连锁（4.6 cM）。RFLP和RAPD标记与所有7个无毒性基因的相引相连锁分别在最小距离0.0 cM和最大距离14.7 cM处被确定。