已分配到现有支架RFLP图谱上的RAPD标记的大豆遗传图谱。

Soybean genetic map of RAPD markers assigned to an existing scaffold RFLP map.

作者信息

Ferreira A R, Foutz K R, Keim P

机构信息

Rigesa Ltda, Dep Florestal, Canoinhas, Brazil.

出版信息

J Hered. 2000 Sep-Oct;91(5):392-6. doi: 10.1093/jhered/91.5.392.

DOI:10.1093/jhered/91.5.392

PMID:10994706

Abstract

A 356-marker linkage map of Glycine max (L.) Merr. (2n = 20) was established by anchoring 106 RAPD markers to an existing RFLP map built with a large recombinant inbred line population (330 RILs). This map comprises 24 major and 11 minor linkage groups for this genome which is estimated to be approximately 3,275 cM. The RAPD markers show similar distribution throughout the genome and identified similar levels of polymorphism as the RFLP markers used in the framework. By using a subset population to anchor the RAPD markers, it was possible to enhance the throughput of selecting and adding reliable marker loci to the existing map. The procedures to generate a dependable genetic linkage map are also described in this report.

摘要

通过将106个随机扩增多态性DNA（RAPD）标记定位到一个用大型重组自交系群体（330个重组自交系）构建的现有限制性片段长度多态性（RFLP）图谱上，构建了大豆（Glycine max (L.) Merr.，2n = 20）的一个包含356个标记的连锁图谱。该图谱包含该基因组的24个主要连锁群和11个次要连锁群，基因组长度估计约为3275厘摩（cM）。RAPD标记在整个基因组中显示出相似的分布，并且与用于构建框架图谱的RFLP标记具有相似水平的多态性。通过使用一个亚群来定位RAPD标记，有可能提高在现有图谱上选择和添加可靠标记位点的通量。本报告还描述了生成可靠遗传连锁图谱的程序。